Q: I would like some details about AGR2 protein. How did you produce this protein? Did you test if it functional on cells?

A: Recombinant human AGR2 protein (NBC1-18502) fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. As far as the applications are concerned, this product can only be used for SDS PAGE and ELISA and to our knowledge it is not suitable for functional assays.

Recombinant Human AG-2/AGR2 His Protein

Novus Biologicals | Catalog # NBC1-18502

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all AG-2/AGR2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-175 of Human AG-2

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSRDTT VKPGAKKDTK DSRPKLPQTL SRGWGDQLIW TQTYEEALYK SKTSNKPLMI IHHLDECPHS QALKKVFAEN KEIQKLAEQF VLLNLVYETT DKHLSPDGQY VPRIMFVDPS LTVRADITGR YSNRLYAYEP ADTALLLDNM KKALKLLKTE L

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AG-2/AGR2 His Protein

SDS-PAGE: Recombinant Human AG-2/AGR2 His Protein [NBC1-18502]

SDS-Page: Recombinant Human AG-2/AGR2 Protein [NBC1-18502] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBC1-18502

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM EDTA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AG-2/AGR2

AGR2(Anterior gradient 2 homolog) is the human orthologue of the secreted Xenopus laevis Anterior Gradient protein(XAG-2). This is a small, possibly secreted molecule of yet weakly defined functions that is widely expressed in human tissues. Expression of AGR2 shows a positive correlation with expression of estrogen receptor in breast carcinoma and a negative correlation with expression of EGF receptor. Recombinant human AGR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Anterior Gradient 2 Homolog

Alternate Names

AG2, AGR2, GOB-4, HPC8, PDIA17

Gene Symbol

AGR2

Additional AG-2/AGR2 Products

Product Documents for Recombinant Human AG-2/AGR2 His Protein

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Product Specific Notices for Recombinant Human AG-2/AGR2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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