Allophycocyanin Antibody (APC-6A2) - BSA Free

Novus Biologicals | Catalog # NBP2-00151

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Non-species specific

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # APC-6A2

Format

BSA Free
View all Allophycocyanin Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is Allophycocyanin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Allophycocyanin Antibody (APC-6A2) - BSA Free

Flow Cytometry: Allophycocyanin Antibody (APC-6A2) [NBP2-00151]

Flow Cytometry: Allophycocyanin Antibody (APC-6A2) [NBP2-00151]

Flow Cytometry: Allophycocyanin Antibody (APC-6A2) [NBP2-00151] - Staining of normal human peripheral blood cells with Rat Anti-Human/Mouse CD44 APC followed by 0.015 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.015 ug of Anti-Allophycocyanin Purified (right) followed by detection with Anti-Mouse IgG FITC. Total viable cells were used for analysis.

Applications for Allophycocyanin Antibody (APC-6A2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
This APC-6A2 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.015 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Allophycocyanin

This antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using APC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. Th oAPC-6A2 can be used for separation of cells labeled with APCconjugated antibodies or for staining.

Alternate Names

APC

Additional Allophycocyanin Products

Product Documents for Allophycocyanin Antibody (APC-6A2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Allophycocyanin Antibody (APC-6A2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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