AP1S2 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP2-14299PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human AP1S2.

Source: E. coli

Amino Acid Sequence: QADLLQEKTETMYHSKSFIGFKKAY

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14299.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP2-14299PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: AP1S2

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where itmediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolictails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one smalladaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of theadaptin protein family. Transcript variants utilizing alternative polyadenylation signals exist for this gene.(provided by RefSeq)

Alternate Names

adaptor-related protein complex 1, sigma 2 subunit, MGC:1902, SIGMA1B, X-linked 59

Gene Symbol

AP1S2

Additional AP1S2 Products

Product Documents for AP1S2 Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for AP1S2 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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