Apc10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30151

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Apc10 Primary Antibodies »

Product Specifications

Immunogen

Between 135 and 185

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Apc10 Antibody - BSA Free (NBP3-30151) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Apc10 Antibody - BSA Free

Apc10 Antibody

Immunoprecipitation:Apc10 AntibodyNBP3-30151] -

Immunoprecipitation:Apc10 AntibodyNBP3-30151] -Detection of human ANAPC10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANAPC10 antibody NBP3-30151 (lot NBP3-30151-1) used for IP at 6 µg per reaction. ANAPC10 was also immunoprecipitated by rabbit anti-ANAPC10.. For blotting immunoprecipitated ANAPC10, A304-980A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Apc10 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Apc10

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Alternate Names

anaphase promoting complex subunit 10, anaphase-promoting complex subunit 10, APC10DOC1, Cyclosome subunit 10, DKFZP564L0562

Gene Symbol

ANAPC10

Additional Apc10 Products

Product Documents for Apc10 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Apc10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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