Home / Other Transferase Inhibitors / C 646

C 646

4200 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4200/10
4200/50
C 646 | CAS No. 328968-36-1 | Other Transferase Inhibitors
1 Image
Description: Selective p300/CBP inhibitor

Chemical Name: 4-[4-[[5-(4,5-Dimethyl-2-nitrophenyl)-2-furanyl]methylene]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzoic acid

Purity: ≥98%

Product Details
Reviews (1)
Biological Activity Technical Data Additional Information Background Product Datasheets Calculators

Biological Activity

C 646 is a selective p300/CREB-binding protein (CBP) inhibitor (Ki = 400 nM). Selective for p300 over six other histone acetyltransferases (HATs). Suppresses histone H3 and H4 acetylation in mouse fibroblast cell lines. Also represses pluripotency markers in mouse embryonic stem cells.

This compound is a mixture of Z:E isomers (~ 80:20).

Technical Data

M.Wt:
445.42
Formula:
C24H19N3O6
Solubility:
Soluble to 25 mM in DMSO
Purity:
≥98%
Storage:
Store at +4°C
CAS No:
328968-36-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Other Product-Specific Information:
  • This compound is a mixture of Z:E isomers (~ 80:20)

Background References

  1. Virtual ligand screening of the p300/CBP histone acetyltransferase: identification of a selective small molecule inhibitor.
    Bowers et al.
    Chem.Biol., 2010;17:471
  2. Butyrate greatly enhances derivation of human induced pluripotent stem cells by promoting epigenetic remodeling and the expression of pluripotency-associated genes.
    Mali et al.
    Stem Cells, 2010;28:713
  3. HAT trick: p300, small molecule, inhibitor.
    Ott and Verdi
    Chem.Biol., 2010;17:417
  4. Dynamic acetylation of all lysine-4 trimethylated histone H3 is evolutionarily conserved and mediated by p300/CBP.
    Crump et al.
    Proc.Natl.Acad.Sci. USA, 2011;108:7814
  5. Roles for histone acetylation in regulation of telomere elongation and two-cell state in mouse ES cells.
    Dan et al.
    J.Cell.Physiol., 2015;230:2337

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Molarity Calculator

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for C 646

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used C 646?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


C 646 In vitro concentration
By Anonymous on 01/08/2018
Application: Species: Human

It is dissolved well in DMSO. I diluted it with serum free medium (final concentration of 10 micromolar) and treated the cells for different time points to detect its inhibitory effect of p300 at protein level.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.