C 646

Tocris Bioscience | Catalog # 4200

p300/CBP inhibitor
Tocris Bioscience
Discontinued Product
4200 has been discontinued. View all Other Transferase Inhibitors products.

Key Product Details

Description

p300/CBP inhibitor
View all Other Transferase Inhibitors »

Product Description

C 646 is a p300/CREB-binding protein (CBP) inhibitor (Ki = 400 nM). Suppresses histone H3 and H4 acetylation in mouse fibroblast cell lines. Also represses pluripotency markers in mouse embryonic stem cells. Newer p300 probes are also available: iP300w (Cat. No. 7270) and A 485 (Cat. No. 6387).

This compound is a mixture of Z:E isomers (~ 80:20).

Product Specifications for C 646

Molecular Weight

445.42

Formula

C24H19N3O6

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

4-[4-[[5-(4,5-Dimethyl-2-nitrophenyl)-2-furanyl]methylene]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzoic acid

CAS Number

328968-36-1

PubChem ID

1285941

InChI Key

HEKJYZZSCQBJGB-UNOMPAQXSA-N

SMILES

CC/2=NN(C(C2=C/C3=CC=C(C4=CC(C)=C(C)C=C4[N+]([O-])=O)O3)=O)C1=CC=C(C(O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.14 25

Preparing Stock Solutions for C 646

The following data is based on the product molecular weight 445.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 8.98 mL 44.90 mL 89.80 mL
1.25 mM 1.80 mL 8.98 mL 17.96 mL
2.5 mM 0.90 mL 4.49 mL 8.98 mL
12.5 mM 0.18 mL 0.90 mL 1.80 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 29 publications citing the usage of this product.

Product Documents for C 646

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for C 646

For research use only

Customer Reviews for C 646 (1)

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  • C 646 In vitro concentration
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Intestinal subepithelial myofibroblasts, smooth muscle cells,
    Verified Customer | Posted 01/08/2018
    It is dissolved well in DMSO. I diluted it with serum free medium (final concentration of 10 micromolar) and treated the cells for different time points to detect its inhibitory effect of p300 at protein level.

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