iP300w

Tocris Bioscience | Catalog # 7270

Potent p300/CBP inhibitor
Tocris Bioscience

Key Product Details

Description

Potent p300/CBP inhibitor
View all Histone Acetyltransferase Inhibitors »

Product Description

iP300w is a potent p300/CBP inhibitor. iP300w inhibits p300-mediated H3K9 acetylation (IC50 = 33 nM). The cytotoxicity of DUX4 and overexpression of DUX4 target genes are inhibited by iP300w in vitro. In addition, the compound inhibits DUX4-mediated transcription in an animal model of facioscapulohumeral muscular dystrophy (FSHD). iP300w reduces viability and inhibits proliferation of CIC-DUX4 sarcoma (CDS) cells in vitro but not pancreatic or colorectal cancer cell lines. The compound suppresses tumor growth in a mouse CDS xenograft model.

iP300w negative control iP300v (Cat. No. 7584) also available.

Product Specifications for iP300w

Molecular Weight

618.55

Formula

C29H27F5N6O4

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(1'S,3'R)-3'-Fluoro-N-[(4-fluorophenyl)methyl]-2',3'-dihydro-5'-[1-[2-(methylamino)-2-oxoethyl]-1H-pyrazol-4-yl]-2,5-dioxo-N-[(1S)-2,2,2-trifluoro-1-methylethyl]spiro[imidazolidine-4,1'-[1H]indene]-1-acetamide

CAS Number

1889284-33-6

PubChem ID

134327298

InChI Key

SWXCHCQCWFXILM-NPPGMGHMSA-N

SMILES

O=C(NC)CN(N=C1)C=C1C(C=C2)=CC3=C2[C@@](N4)(C[C@H]3F)C(N(CC(N(CC5=CC=C(F)C=C5)[C@@H](C)C(F)(F)F)=O)C4=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 61.85 100

Preparing Stock Solutions for iP300w

The following data is based on the product molecular weight 618.55.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.62 mL 8.08 mL 16.17 mL
5 mM 0.32 mL 1.62 mL 3.23 mL
10 mM 0.16 mL 0.81 mL 1.62 mL
50 mM 0.03 mL 0.16 mL 0.32 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for iP300w

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for iP300w

For research use only

Related Research Areas

Epigenetics

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