Calcein AM: R&D Systems
Catalog Number: 5119
Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
Biological Activity
Cell permeable, non fluorescent compound; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells. Used for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.
Technical Data
  • M.Wt:
    994.86
  • Formula:
    C46H46N2O23
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
    >95%
  • Storage:
    Store at -20°C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation.
    Lazarowski et al.
    J.Biol.Chem., 1997;272:24328
  2. Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release.
    Kuehn et al.
    J.Biol.Chem., 2011;286:391
  3. The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay.
    De Gendt et al.
    Clin.Chim.Acta., 1996;249:189
  4. Membrane topology and glycosylation of the human multidrug resistance-associated protein.
    Bakos et al.
    J.Biol.Chem., 1996;271:12322

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