Calcein AM

Catalog # Availability Size / Price Qty
5119/1
Calcein AM | CAS No. 890090-35-4 | Cell Viability Stains and Dyes
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Description: Cell permeable compound; hydrolyzed to become fluorescent in living cells

Chemical Name: N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester

Purity: ≥90%

Product Details
Citations (5)
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Biological Activity

Calcein AM is a cell permeable, non-fluorescent compound for monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance; hydrolyzed by intracellular esterases to become fluorescent calcein in living cells.

It is recommended to prepare stock solutions of Calcein AM in DMSO.

Optical Data for Calcein AM

Calcein AM Dye Spectra
Emission Color Green
λabs 495 nm
λem 515 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescent Microscopy

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
994.86
Formula:
C46H46N2O23
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
890090-35-4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Citations for Calcein AM

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