CD45RC Antibody (OX-22) [FITC]

Novus Biologicals | Catalog # NB100-63850

Novus Biologicals
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-22
View all CD45RC Primary Antibodies »

Product Specifications

Immunogen

Phytohaemagglutinin (PHA) -activated rat lymphocytes

Localization

Cell Membrane

Specificity

NB120-6406 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. This antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations,CD4+ CD45RC (Th 1-like) and CD4+ CD45RC low (Th-2 like). This product is routinely tested in flow cytometry on rat splenocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD45RC Antibody (OX-22) [FITC]

Flow Cytometry: CD45RC Antibody (OX22) [FITC] [NB100-63850] - Staining of rat spleen cells with MOUSE.ANTI RAT CD45RC:FITC

Applications for CD45RC Antibody (OX-22) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1% Bovine Serum Albumin

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD45RC

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

P04157 (Rat)

Additional CD45RC Products

Product Documents for CD45RC Antibody (OX-22) [FITC]

Certificate of Analysis

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Product Specific Notices for CD45RC Antibody (OX-22) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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