Recombinant Human CDC2/CDK1 His Protein

Novus Biologicals | Catalog # NBP2-51886

Novus Biologicals
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Key Product Details

Source

Baculovirus

Tag

His

Applications

SDS-PAGE
View all CDC2/CDK1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-297 of Human CDC2/CDK1

Source: Baculovirus

Amino Acid Sequence: MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMzLEH HHHHH

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

35.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CDC2/CDK1 His Protein

SDS-PAGE: Recombinant Human CDC2/CDK1 His Protein [NBP2-51886]

SDS-PAGE: Recombinant Human CDC2/CDK1 His Protein [NBP2-51886]

SDS-Page: CDC2/CDK1 Recombinant Protein [NBP2-51886] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-51886
Formulation PBS (pH 7.4), 30% glycerol, 2 mM DTT, 0.1 mM PMSF
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol

CDK1

Additional CDC2/CDK1 Products

Product Documents for Recombinant Human CDC2/CDK1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human CDC2/CDK1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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