CpG oligodeoxynucleotides Mouse

Novus Biologicals | Catalog # NBP2-26235

Novus Biologicals
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Key Product Details

Species

Mouse

Applications

Functional Assay

Product Summary for CpG oligodeoxynucleotides Mouse

Mouse Sequence CpG ODN (1826) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*C*G*T*T 3

(* Indicates a phosphorothioate modification)

Negative Control oligo: 5' TCCATGAGCTTCCTGAGCTT 3'

Functionality: This product is useful for the activation of TLR9 and the stimulation of TLR9 has been reported with 5-20 ug/ml.

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Product Specifications

Specificity

CpG ODN (1826) with negative control oligo, TLR9 ligand (mouse)

Applications

Ligand Activation (5-20 ug/ml)

Application Notes

A TLR9/NF-kB SEAP reporter construct in a HEK 293 cell line was used as a model system for studying hTLR9 activation.

Scientific Data Images for CpG oligodeoxynucleotides Mouse

CpG oligodeoxynucleotides Mouse [NBP2-26235] - CpG oligodeoxynucleotides Mouse Evaluation of the mCpG ODN 1826 ligand activity on NF-kB SEAPorter RAW cell line. Cell line is a stably transfected RAW 264.7 cell line that expresses the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. RAW 264.7 cells endogenously express Toll-like receptor 9 (TLR9). Cells were plated in 96-well plates at 0.85 x 10^5 cells/well. After 16 h, cells were stimulated with various amounts of mCpG ODN 1826 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: mCpG ODN 1826 specifically activated the TLR9-depedent NF-kB/SEAP reporter cells in a dose dependent manner.

Formulation, Preparation, and Storage

Formulation

Sterile water

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CpG oligodeoxynucleotides Mouse

Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motifs. These CpG motifs are present at a 20 fold greater frequency in bacterial DNA than mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9). Two types of CpG ODNs have been identified based on their distinct activity on plasmacytoid dendritic cells (pDC), which is the key sensors of the CpG motifs. CpG Type A induces IFN-a production in pDC whereas Type B strongly activates B cells and weakly activates IFN-a stimulation. The CpG ODN sequence differs between human and mouse, however both negative controls contain GpC instead of CpG.

Alternate Names

CpG oligodeoxynucleotides (ODN), ODN

Additional CpG oligodeoxynucleotides Mouse Products

Product Documents for CpG oligodeoxynucleotides Mouse

Certificate of Analysis

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Product Specific Notices for CpG oligodeoxynucleotides Mouse

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for CpG oligodeoxynucleotides Mouse

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Protocols

View specific protocols for CpG oligodeoxynucleotides Mouse (NBP2-26235):

CpG oligodeoxynucleotides Mouse:
Hazard Information
Chemical Name: ODN 1826 & Negative Control
Chemical Formula: CpG ODN- 5' TCCATGACGTTCCTGACGTT 3' (in bold is phosphorothiate linkage)
CAS Number: N/A
EEC-No: N/A

First Aid Measures
Eye Contact: May cause eye irritation.
Skin Contact: May cause skin irritation. May be harmful if absorbed through the skin.
Inhalation: May causes respiratory tract irritation. May be harmful if inhaled.
Ingestion: May be harmful if swallowed.

Fire Fighting Measures
Special risks: N/A
Suitable Extinguish Media: Water spray, carbon dioxide, dry chemical powder or appropriate foam.

Accidental Release Measures
Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Consult a physician.
Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. SPEEDY ACTION IS CRITICAL!
Ingestion: If swallowed, get medical aid immediately. Only induce vomiting if directed to do so by medical personnel. Never give anything by mouth to an unconscious person.
Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. SPEED IS ESSENTIAL, OBTAIN MEDICAL AID IMMEDIATELY. Do not use mouth-to-mouth resuscitation if victim ingested or inhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device.
Notes: Consult a physician. Show this safety data sheet to the physician. Move away from the dangerous area.

Handling and Storage
Handling: Avoid contact with eyes, skin and clothing. Avoid prolonged or repeated exposure. Avoid inhalation. Use personal protective equipment (i.e. impermeable gloves, lab coat or apron).
Store at -20 degrees C. Store in a tightly closed container. Stable under recommended storage conditions

Exposure Controls / Personal Protection
Physical and Chemical Properties
Form: Powder
Color: Colorless
Odor: Odorless
Melting Point: No data available
Boiling Temperature: No data available
Density: No data available
Vapor Pressure: No data available
Solubility in Water: Very soluble
Flash Point: No data available
Explosion limits: No data available
Ignition Temperature: No data available

Stability and Reactivity
Stability: Stable
Hazardous Polymerization: Will not occur.
Materials to avoid: Strong oxidizing agents.
Hazardous Decomposition Products: Decomposition products are not hazardous.
HMIS Classification: Health Hazard 0, Flammability Hazard 0, Reactivity Hazard 0
NFPA Rating: Health Hazard 0, Fire 0, Reactivity Hazard 0.

Disposal Considerations
Observe all federal, state, and local environmental regulations. Contact a licensed professional waste disposal service to dispose of this material. Must not be disposed together with household garbage.

Other Information
The information contained in this material safety datasheet is believed to be accurate but it is the responsibility of the user or supplier to determine the applicability of these data to the formulation of necessary safety precautions.
NOVUS shall not be held responsible for any damage resulting from the use of the above product or the information contained in this material safety data sheet.

FAQs

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