Cultrex Mouse Collagen IV

2 vials (0.5 mg/vial) of Cultrex Mouse Collagen IV
Catalog # Availability Size / Price Qty
3410-010-02
Product Details
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Cultrex Mouse Collagen IV Summary

Cultrex Mouse Collagen IV is a purified stromal extracellular matrix protein that can promtes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.

Key Benefits

• Qualified for general cell culture applications
• May be used as a hydrogel or thin coating for tissue culture vessels
• 1 mg of material is provided in 2 vials (0.5 mg/vial)

Why Use Cultrex Mouse Collagen IV?

Collagen IV is a major component of basement membranes, which underlies epithelial and endothelial cells and surrounds muscle, fat and nerve cells. It can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior.

Intended Use

Cultrex Mouse Collagen IV is used as a substrate for cell culture adhesion. The recommended working concentration is 1-10 μg/cm^2 (1-10 μg/mL) of growth surface depending on cell type. Cultrex Mouse Collagen IV is supplied as 2 vials each containing 0.5 mg of protein.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Tested for ability to promote attachment and spreading of rat pheochromocytoma (PC-12) cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Stability
Product is stable for 3 months from date of shipment when stored at ≤ -70 °C. See lot specifc Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Product is stable for 3 months from date of shipment when stored at -70 °C.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

FAQs

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