Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
Cultrex Mouse Collagen IV
R&D Systems | Catalog # 3410-010-02
2 vials (0.5 mg/vial) of Cultrex Mouse Collagen IV (Catalog # 3410-010-01)
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Key Product Details
Features
Cultrex Mouse Collagen IV is a purified stromal extracellular matrix protein that can promote cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.
Key Benefits
- Qualified for general cell culture applications
- May be used as a hydrogel or thin coating for tissue culture vessels
- 1 mg of material is provided in 2 vials of Catalog # 3410-010-01 at 0.5 mg/vial
Species
Mouse
Product Summary for Cultrex Mouse Collagen IV
Why Use Cultrex Mouse Collagen IV?
Collagen IV is a major component of basement membranes, which underlies epithelial and endothelial cells and surrounds muscle, fat and nerve cells. It can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior.
Negative for the presence of bacteria and fungi.
Negative for the presence of bacteria and fungi.
Intended Use
Cultrex Mouse Collagen IV is used as a substrate for cell culture adhesion. The recommended working concentration is 1-10 μg/cm^2 (1-10 μg/mL) of growth surface depending on cell type. Cultrex Mouse Collagen IV (3410-010-02) is supplied as 2 vials of (3410-010-01) each containing 0.5 mg of protein.
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Product Specifications
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
Tested for ability to promote attachment and spreading of rat pheochromocytoma (PC-12) cells.
Viral Testing
No mycoplasma contamination detected by PCR.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Product is stable for 3 months from date of shipment when stored at -70 °C.
Stability
Product is stable for 3 months from date of shipment when stored at ≤ -70 °C. See lot specifc Certificate of Analysis for expiration date.
Background: Collagen I
Alternate Names
Alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, Collagen 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, Type I Procollagen Alpha 1 Chain
Additional Collagen I Products
Product Documents for Cultrex Mouse Collagen IV
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Citations for Cultrex Mouse Collagen IV
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