Q: I would like to find out if this enzymes DAO Protein (NBC1-28774) are functional and have been tested for enzyme activities.

A: In regards to your inquiry about our DAO recombinant protein (NBC1-28774), unfortunately this is not expected to be active and has not been tested for activity by the lab. Therefore we can neither guarantee or not guarantee that it will be functional or that would be listed as an application on the datasheet.

Recombinant Human DAO His Protein

Novus Biologicals | Catalog # NBC1-28774

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all D-Amino Acid Oxidase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-347 of Human DAO

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DAO His Protein

SDS-PAGE: Recombinant Human DAO His Protein [NBC1-28774]

SDS-Page: Recombinant Human DAO Protein [NBC1-28774] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBC1-28774

Formulation	20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: D-Amino Acid Oxidase

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

D-Amino Acid Oxidase

Alternate Names

DAAO, DAMOX, DAO, OXDA

Gene Symbol

DAO

Additional D-Amino Acid Oxidase Products

Product Documents for Recombinant Human DAO His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human DAO His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human DAO His Protein

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