DCI Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP1-91822PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
View all DCI Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ECI1.

Source: E. coli

Amino Acid Sequence: FGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRS

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-91822.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP1-91822PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: DCI

The DCI gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising durin

Alternate Names

acetylene-allene isomerase, D2-enoyl-CoA isomerase, D3, DCI3,2-trans-enoyl-CoA isomerase3,2 trans-enoyl-CoA isomerase, Delta(2)-enoyl-CoA isomerase, Delta(3), delta3, delta2-enoyl-CoA isomerase, dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase), dodecenoyl-CoA isomerase3,2-trans-enoyl-CoA isomerase, mitochondrial, dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase), EC 5.3.3.8, enoyl-CoA delta isomerase 13,2 trans-enoyl-Coenzyme A isomerase

Gene Symbol

ECI1

Additional DCI Products

Product Documents for DCI Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for DCI Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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