Recombinant Human DNA Polymerase epsilon subunit 3 His Protein

Name: Recombinant Human DNA Polymerase epsilon subunit 3 His Protein
Brand: Novus Biologicals
SKU: NBP2-23341
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-23341

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all DNA Polymerase epsilon subunit 3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-147 of Human DNA Polymerase epsilon subunit 3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DNA Polymerase epsilon subunit 3 His Protein

SDS-PAGE: Recombinant Human DNA Polymerase epsilon subunit 3 His Protein [NBP2-23341]

SDS-Page: Recombinant Human DNA Polymerase epsilon subunit 3 His Protein [NBP2-23341] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP2-23341

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Polymerase epsilon subunit 3

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. POLE3 has been shown to interact with SMARCA5. Recombinant human POLE3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Arsenic-transactivated protein, AsTP, CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1

Gene Symbol

POLE3

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Certificate of Analysis

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Product Specific Notices for Recombinant Human DNA Polymerase epsilon subunit 3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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