DuoSet ELISA Ancillary Reagent Kit 3

Newer Version Available: DY009B
Product in process of being discontinued and replaced by new Next generation kit

Discontinued Product

DY009 has been discontinued and is replaced by DY009B.

R&D Systems DuoSet Ancillary Reagent Kits
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Product Details
Citations (1)
Reviews (6)

DuoSet ELISA Ancillary Reagent Kit 3 Summary

Assay Type:ELISA Development Kit
Sufficient Materials:For five 96-well plate assays

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 3 contains Reagent Diluent 3. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.

  • Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 5% Tween® 20 in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

  • Clear Polystyrene Microplates (five 12x8-well strip microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • Color Reagent A (1X, three 12.5 mL vials)
  • Color Reagent B (1X, three 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • 20X PBS
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 3 (5X, three 21 mL vials of a 25% Tween 20 solution in PBS)
  • Wash Buffer (25X, six 21 mL vials)


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citation for DuoSet ELISA Ancillary Reagent Kit 3

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. SIRT1 suppresses in vitro decidualization of human endometrial stromal cells through the downregulation of forkhead box O1 expression
    Authors: SL Yu, SI Lee, HW Park, SK Lee, TH Kim, J Kang, SR Park
    Oncogene, 2022;22(3):100672.  2022


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Reviews for DuoSet ELISA Ancillary Reagent Kit 3

Average Rating: 4.8 (Based on 6 Reviews)

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DuoSet ELISA Ancillary Reagent Kit 3
By Anonymous on 03/29/2022

Very good kit. Works well with inserts supplied as well as with other high binding plates.

DuoSet ELISA Ancillary Reagent Kit 3
By Anonymous on 10/01/2020

I don't use this as often as the reagent kit 2 but it works just as well and produces great results.

DuoSet ELISA Ancillary Reagent Kit 3
By Jonatan Dereke on 08/06/2019

The human IGFBP-3 and IGFBP-5 DuoSet ELISA kits have been run with this Ancillary kit.

DuoSet ELISA Ancillary Reagent Kit 3
By Anonymous on 07/25/2018

DuoSet ELISA Ancillary Reagent Kit 3
By Leslie Priddy on 04/03/2018

DuoSet ELISA Ancillary Reagent Kit 3
By Anonymous on 05/12/2017