EV-FACS (Cell Culture Media) Flow Cytometry Kit
Novus Biologicals | Catalog # NBP3-18363
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Key Product Details
Species
Human
Applications
Flow Cytometry
Kit Type
Flow Cytometry Kit
Product Summary for EV-FACS (Cell Culture Media) Flow Cytometry Kit
Streamline EV Flow Cytometry with EV-FACS. Everything needed to isolate and analyze extracellular vesicles by flow cytometry including: EV Precipitation Solution, 4 um beads for capture and exosome standards. One primary antibody (CD63 or CD9) included as positive control with flexibility to add other antibodies for multiplex analysis.
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Product Specifications
Sample Volume
1-5 mL
Scientific Data Images for EV-FACS (Cell Culture Media) Flow Cytometry Kit
Flow Cytometry: EV-FACS (Cell Culture Media) Flow Cytometry Kit [NBP3-18363] -
Flow Cytometry: EV-FACS (Cell Culture Media) Flow Cytometry Kit [NBP3-18363] - FACS profiling of exosomal markers CD9, CD63 and TM9SF4 in purified exosomes from Melanoma (MM1), Neuroblastoma (SH) and Glioblastoma (U87) cell supernatants. Exosome binding on FACS-beads was performed by incubation at 4C overnight.Kit Contents for EV-FACS (Cell Culture Media) Flow Cytometry Kit
- EXO-Prep
- FACS-beads
- Lyophilized Exosome Standards
- Primary Antibody
- Sample Buffer (5x)
- Secondary Antibody Alexa Fluor 488
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C. Do not freeze.
Product Documents for EV-FACS (Cell Culture Media) Flow Cytometry Kit
Certificate of Analysis
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Product Specific Notices for EV-FACS (Cell Culture Media) Flow Cytometry Kit
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
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- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
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- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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