EV MiniSEC Columns

Name: EV MiniSEC Columns
Brand: Novus Biologicals
SKU: NBP3-41043
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-41043

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Product Summary for EV MiniSEC Columns

PURE-EVs are size exclusion chromatography (SEC) columns designed to isolate extracellular vesicles (EVs) from small volume samples. Compared to other techniques, PURE-EV efficiently separates EVs from circulating proteins while maintaining original shape and functionality of vesicles. Highly pure EV preparations, no residual chemicals from precipitation, and rapid isolation in approximately 20 minutes. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.

Product Specifications

Sample Volume

100 uL - 500 uL

Scientific Data Images for EV MiniSEC Columns

EV MiniSEC Columns [NBP3-41043]

EV MiniSEC Columns [NBP3-41043] - EV separation - EV MiniSEC Column was filled with 500 uL of medium from HCT116 cells, previously concentrated by TFF-Easy. 20 fractions (100 μl each one) have been collected and analyzed by ELISA assay (CD81 marker) and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 9 - 14 (turnaround time approximately 10 min), whereas circulating proteins corresponded to the fractions 16 - 20.

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C.

Background: EV MiniSEC Columns

Size-exclusion chromatography (SEC) separates macromolecules based on their size. To accomplish this, the sample is passed through a porous column and molecules are isolated in fractions from the eluate. The size of the pores can vary depending on the size of the desired product. For isolation of EVs from proteins and lipoproteins, pore sizes can vary from ~40-80 nm. As the sample passes through the column, molecules smaller than the pore size will get trapped in the pores, slowing them down, while larger molecules will pass through. This results in the larger molecules, such as EVs, eluting from the column in earlier fractions, while smaller molecules, such as proteins and protein aggregates, are eluted in later fractions.

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Product Documents for EV MiniSEC Columns

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Certificate of Analysis

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Product Specific Notices for EV MiniSEC Columns

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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