Recombinant Human EVI-1 His Protein

Name: Recombinant Human EVI-1 His Protein
Brand: Novus Biologicals
SKU: NBP2-22647
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-22647

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all EVI-1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-169 of Human EVI-1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMRSKGRA RKLATNNECV YGNYPEIPLE EMPDADGVAS TPSLNIQEPC SPATSSEAFT PKEGSPYKAP IYIPDDIPIP AEFELRESNM PGAGLGIWTK RKIEVGEKFG PYVGEQRSNL KDPSYGWEVH LPRSRRVSVH SWLYLGKRSS DVGIAFSQAD VYMPGLQCAF LS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EVI-1 His Protein

SDS-PAGE: Recombinant Human EVI-1 His Protein [NBP2-22647]

SDS-Page: EVI1 Protein [NBP2-22647]

Formulation, Preparation, and Storage

NBP2-22647

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EVI-1

MDS1 is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. Recombinant human MDS1 protein, fused to His-tag at N-terminus, was expressed in E.coli

Long Name

Ecotropic Viral Integration Site 1

Alternate Names

EVI1, MDS1, MECOM, PRDM3

Gene Symbol

MECOM

Additional EVI-1 Products

Product Documents for Recombinant Human EVI-1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human EVI-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Hematopoietic Stem Cell Markers

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