FlowTACS Flow Cytometry Apoptosis Detection Kit

Identification and quantitation of fixed apoptotic cells in suspension by Flow Cytometry
Catalog # Availability Size / Price Qty
4817-60-K
FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K
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Product Details
Citations (9)
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FlowTACS Flow Cytometry Apoptosis Detection Kit Summary

Provides an in situ labeling technology to identify and quantitate cultured apoptotic cells by TUNEL-based and flow cytometry.

Key Benefits

• Identification and quantitation of fixed apoptotic cells in suspension by flow cytometry.
• Allows bi-color and tri-color analysis.
• Designed for use with fixed cells.
• Includes exclusive non-enzymatic Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Why Use the FlowTACS Flow Cytometry Apoptosis Detection Kit?

FlowTACS also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.

Kit Contents

• Streptavidin-FITC
• TACS-Nuclease
• TACS-Nuclease Buffer
• /TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• 10X TdT Labeling Buffer
• 10X TdT Stop Buffer
• PI/RNase
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Data Example

Flow Cytometry FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K View Larger

Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes. Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.

Citations for FlowTACS Flow Cytometry Apoptosis Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro
    Authors: X Wang, M Bai
    BMC cardiovascular disorders, 2021;21(1):51.  2021
  2. Targeting IL-17A/glucocorticoid synergy to CSF3 expression in neutrophilic airway diseases
    Authors: S Ouyang, C Liu, J Xiao, X Chen, AC Lui, X Li
    JCI Insight, 2020;5(3):.  2020
  3. Platelet-derived growth factor-C (PDGF-C) induces anti-apoptotic effects on macrophages through Akt and Bad phosphorylation.
    Authors: Son D, Na Y, Hwang E, Seok S
    J Biol Chem, 2014;289(9):6225-35.  2014
  4. The TACI receptor regulates T-cell-independent marginal zone B cell responses through innate activation-induced cell death.
    Authors: Figgett W, Fairfax K, Vincent F, Le Page M, Katik I, Deliyanti D, Quah P, Verma P, Grumont R, Gerondakis S, Hertzog P, O'Reilly L, Strasser A, Mackay F
    Immunity, 2013;39(3):573-83.  2013
  5. Theiler's virus-induced intrinsic apoptosis in M1-D macrophages is Bax mediated and restricts virus infectivity: a mechanism for persistence of a cytolytic virus.
    Authors: Son KN, Becker RP, Kallio P, Lipton HL
    J. Virol., 2008;82(9):4502-10.  2008
  6. Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling.
    Authors: Wang L, Schulz TC, Sherrer ES, Dauphin DS, Shin S, Nelson AM, Ware CB, Zhan M, Song CZ, Chen X, Brimble SN, McLean A, Galeano MJ, Uhl EW, D'Amour KA, Chesnut JD, Rao MS, Blau CA, Robins AJ
    Blood, 2007;110(12):4111-9.  2007
  7. Butyrate-induced apoptosis and cell cycle arrest in bovine kidney epithelial cells: involvement of caspase and proteasome pathways.
    Authors: Li CJ, Elsasser TH
    J. Anim. Sci., 2005;83(1):89-97.  2005
  8. Division-linked generation of death-intermediates regulates the numerical stability of memory CD8 T cells.
    Authors: Nolz J, Rai D, Badovinac V, Harty J
    Proc Natl Acad Sci U S A, 0;109(16):6199-204.  0
  9. Insight into the mechanism of action of temporin-SHa, a new broad-spectrum antiparasitic and antibacterial agent.
    Authors: Raja Z, Andre S, Abbassi F, Humblot V, Lequin O, Bouceba T, Correia I, Casale S, Foulon T, Sereno D, Oury B, Ladram A
    PLoS ONE, 0;12(3):e0174024.  0

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