FlowTACS Flow Cytometry Apoptosis Detection Kit
R&D Systems | Catalog # 4817-60-K
Identification and quantitation of fixed apoptotic cells in suspension by Flow Cytometry.
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Key Product Details
Features
Provides an in situ labeling technology to identify and quantitate cultured apoptotic cells by TUNEL-based and flow cytometry.
Key Benefits
- Identification and quantitation of fixed apoptotic cells in suspension by flow cytometry.
- Allows bi-color and tri-color analysis.
- Designed for use with fixed cells.
- Includes exclusive non-enzymatic Cytonin permeabilization reagent.
- Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
Species
Multi-Species
Product Summary for FlowTACS Flow Cytometry Apoptosis Detection Kit
Why Use the FlowTACS Flow Cytometry Apoptosis Detection Kit?
FlowTACS also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.
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Scientific Data Images for FlowTACS Flow Cytometry Apoptosis Detection Kit
Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes.
Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.Kit Contents for FlowTACS Flow Cytometry Apoptosis Detection Kit
- Streptavidin-FITC
- TACS-Nuclease
- TACS-Nuclease Buffer
- /TACS 2 TdT dNTP
- TdT Enzyme
- 50x Manganese Cation
- 10X TdT Labeling Buffer
- 10X TdT Stop Buffer
- PI/RNase
- Cytonin
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for FlowTACS Flow Cytometry Apoptosis Detection Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Citations for FlowTACS Flow Cytometry Apoptosis Detection Kit
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