FlowTACS Flow Cytometry Apoptosis Detection Kit

Identification and quantitation of fixed apoptotic cells in suspension by Flow Cytometry
Catalog # Availability Size / Price Qty
4817-60-K
FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K
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FlowTACS Flow Cytometry Apoptosis Detection Kit Summary

Provides an in situ labeling technology to identify and quantitate cultured apoptotic cells by TUNEL-based and flow cytometry.

Key Benefits

• Identification and quantitation of fixed apoptotic cells in suspension by flow cytometry.
• Allows bi-color and tri-color analysis.
• Designed for use with fixed cells.
• Includes exclusive non-enzymatic Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Why Use the FlowTACS Flow Cytometry Apoptosis Detection Kit?

FlowTACS also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.

Kit Contents

• Streptavidin-FITC
• TACS-Nuclease
• TACS-Nuclease Buffer
• /TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• 10X TdT Labeling Buffer
• 10X TdT Stop Buffer
• PI/RNase
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Flow Cytometry FlowTACS Flow Cytometry Apoptosis Detection Kit_4817-60-K View Larger

Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes. Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.

Reagents Provided

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