FlowTACS Flow Cytometry Apoptosis Detection Kit
FlowTACS Flow Cytometry Apoptosis Detection Kit Summary
• Identification and quantitation of fixed apoptotic cells in suspension by flow cytometry.
• Allows bi-color and tri-color analysis.
• Designed for use with fixed cells.
• Includes exclusive non-enzymatic Cytonin permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
Why Use the FlowTACS Flow Cytometry Apoptosis Detection Kit?
FlowTACS also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.
• TACS-Nuclease Buffer
• /TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• 10X TdT Labeling Buffer
• 10X TdT Stop Buffer
For research use only. Not for diagnositic use.
Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes. Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.
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