Antineoplastic antimetabolite. Exhibits antiproliferative activity; inhibits thymidylate synthetase and disrupts DNA replication in human cells. Induces double-strand DNA breaks; activates ATR and ATM signaling pathways. Induces phosphorylation of Chk1 and Chk2 in OVCAR-8 and SKOV3ip ovarian cancer cell lines.
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All Tocris products are intended for laboratory research use only.
Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells.
Huehls AM, Wagner JM, Huntoon CJ et al.
Human leukemic cells resistant to 5-fluoro-2'-deoxyuridine contain a thymidylate synthetase with lower affinity for nucleotides.
Bapat et al.
Participation of DNA repair in the response to 5-fluorouracil.
Wyatt and Wilson
Cell.Mol.Life Sci., 2009;66:788