Fluorescent Exosome Standards (HT29 cell line)

Novus Biologicals | Catalog # NBP3-41040

Novus Biologicals
100% Guarantee

Key Product Details

Applications

Flow Cytometry, Microscopy

Product Summary for Fluorescent Exosome Standards (HT29 cell line)

Highly pure fluorescent exosome standards with superior performance, suitable for extracellular vesicle (EV) tracking studies, flow cytometry, and electron microscopy.

One vial contains 100 ug of purified exosomes (measured as total protein content; number of particles in 100 ug: > 1x10^10). Fluorescent labeled exosomes are stable for approximately 6 months storage at -20C. Avoid repeated freeze-and-thaw cycles. Protect from light

Product Specifications

Application Notes

The excitation maximum of fluorescent exosome standards is 500 nm - 650 nm and emission maximum is 510 - 665 nm.
Membrane lipid dye (penetrates into exosome membrane).

Scientific Data Images for Fluorescent Exosome Standards (HT29 cell line)

Fluorescent Exosome Standards (HT29 cell line)

Fluorescent Exosome Standards (HT29 cell line) [NBP3-41040]

Fluorescent Exosome Standards (HT29 cell line) [NBP3-41040] - Absorption and corrected fluorescence emission spectrum of conjugate excitation at 488 nm. Excitation spectrum (dotted line) and emission spectrum (solid line).

Formulation, Preparation, and Storage

Preparation Method

Exosome isolation involves a combination of ultracentrifugation and microfiltration procedures. Fluorescent exosomes are subsequently quantified and validated for overall protein content and particle number by Nanoparticles Tracking Analysis.

Formulation

Cell culture media

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: Fluorescent Exosome Standards (HT29 cell line)

Exosomes are small endosome derived lipid nanoparticles (30-150nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Additional Fluorescent Exosome Standards (HT29 cell line) Products

Product Documents for Fluorescent Exosome Standards (HT29 cell line)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluorescent Exosome Standards (HT29 cell line)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Customer Reviews for Fluorescent Exosome Standards (HT29 cell line)

There are currently no reviews for this product. Be the first to review Fluorescent Exosome Standards (HT29 cell line) and earn rewards!

Have you used Fluorescent Exosome Standards (HT29 cell line)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Supplemental Kits and Reagents