Q: hi I was wondering if the recombinant human galectin-1 protein (NBP2-34878-100ug) has a tag on it?

Recombinant Human Galectin-1 Protein

Novus Biologicals | Catalog # NBP2-34878

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Accession Number

P09382

Applications

Bioactivity, SDS-PAGE

View all Galectin-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 134 amino acids corresponding to Galectin-1 Source: E. coli

Uniprot ID: P09382

Amino Acid Sequence: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Galectin-1 as determined by LAL method.

Predicted Molecular Mass

14.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Galectin 1 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Galectin-1 Protein

SDS-PAGE: Recombinant Human Galectin-1 Protein [NBP2-34878]

SDS-Page: Human Galectin-1 Protein [NBP2-34878]

Formulation, Preparation, and Storage

NBP2-34878

Formulation	Lyophilized from a 0.2 um filtered solution in PBS pH 7.0, 3mM beta-Me, 0.02% Tween 20.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Galectin-1

Human Galectin-1 also named BHL, Galaptin, GBP, L-14 and LGALS1, is belonging to the galectins family and it is encoded by the LGALS1 gene in human. Galectin-1 is expressed by the endometrial stromal cells throughout the menstrual cycle. Galectin-1 contains a single carbohydrate recognition domain through which it can bind glycans both as a monomer and as a homodimer. Dimers are non-covenantly bound and will spontaneously disassociate in low concentration. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 is thought to play a role in the immunosuppression required for a successful pregnancy. Human Galectin-1 shares 88 %-90 % amino acid sequence identity with rat and mouse.

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Gene Symbol

LGALS1

UniProt

P09382

Additional Galectin-1 Products

Product Documents for Recombinant Human Galectin-1 Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Galectin-1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis

Lectins

NKG2D Receptors and Ligands

Regulatory T Cells (Tregs)

Citations for Recombinant Human Galectin-1 Protein

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