HT gamma -H2AX Pharmacodynamic Assay
HT gamma -H2AX Pharmacodynamic Assay Summary
• Chemiluminescent, non-radioactive ELISA
• Pre-coated 96 well capture antibody plate
• Dynamic range from 10 pM to 800pM
• Sensitivity with 5 pM of gamma-H2AX
Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.
• Gamma-H2AX Standard, 1 µM
• Jurkat Cell Lysate Control
• Assay Buffer
• Cell Lysis Reagent
• 25X Wash Buffer
• 96-Stripwell pre-coated plate with 3 film sealers
• PARP PeroxyGlow A
• PARP PeroxyGlow B
• H2AX IgM Detecting Antibody
• Goat anti-Mouse IgM HRP Conjugate
For research use only. Not for diagnositic use.
Citations for HT gamma -H2AX Pharmacodynamic Assay
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Improved Therapeutic Window in BRCA-mutant Tumors with Antibody-linked Pyrrolobenzodiazepine Dimers with and without PARP Inhibition
Authors: H Zhong, C Chen, R Tammali, S Breen, J Zhang, C Fazenbaker, M Kennedy, J Conway, BW Higgs, N Holoweckyj, R Raja, J Harper, AJ Pierce, R Herbst, DA Tice
Mol. Cancer Ther., 2019;18(1):89-99. 2019
The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B)
Authors: P Rossner, T Cervena, M Vojtisek-L, K Vrbova, A Ambroz, Z Novakova, F Elzeinova, H Margaryan, V Beranek, M Pechout, D Macoun, J Klema, A Rossnerova, M Ciganek, J Topinka
Int J Mol Sci, 2019;20(22):. 2019
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