HT gamma -H2AX Pharmacodynamic Assay

For the quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies
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Product Details
Citations (2)

HT gamma -H2AX Pharmacodynamic Assay Summary

Enables the quatification of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies.

Key Benefits

• Chemiluminescent, non-radioactive ELISA
• Pre-coated 96 well capture antibody plate
• Dynamic range from 10 pM to 800pM
• Sensitivity with 5 pM of gamma-H2AX

Product Specifications

Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.

Other Resources


Kit Contents

• Gamma-H2AX Standard, 1 µM
• Jurkat Cell Lysate Control
• Assay Buffer
• Cell Lysis Reagent
• 25X Wash Buffer
• 96-Stripwell pre-coated plate with 3 film sealers
• PARP PeroxyGlow A
• PARP PeroxyGlow B
• H2AX IgM Detecting Antibody
• Goat anti-Mouse IgM HRP Conjugate


Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnositic use.

Product Datasheets

Citations for HT gamma -H2AX Pharmacodynamic Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Improved Therapeutic Window in BRCA-mutant Tumors with Antibody-linked Pyrrolobenzodiazepine Dimers with and without PARP Inhibition
    Authors: H Zhong, C Chen, R Tammali, S Breen, J Zhang, C Fazenbaker, M Kennedy, J Conway, BW Higgs, N Holoweckyj, R Raja, J Harper, AJ Pierce, R Herbst, DA Tice
    Mol. Cancer Ther., 2019;18(1):89-99.  2019
  2. The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B)
    Authors: P Rossner, T Cervena, M Vojtisek-L, K Vrbova, A Ambroz, Z Novakova, F Elzeinova, H Margaryan, V Beranek, M Pechout, D Macoun, J Klema, A Rossnerova, M Ciganek, J Topinka
    Int J Mol Sci, 2019;20(22):.  2019


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