Human CA125/MUC16 Alexa Fluor® 647-conjugated Antibody Summary
Met13360-Gln14347 (Met13472Thr & Gln13957Lys)
Accession # NP_078966.2
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CA125/MUC16 in A431 Human Cell Line by Flow Cytometry. A431 human epidermoid carcinoma cell line was stained with Mouse Anti-Human CA125/MUC16 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB5609R, filled histogram) or isotype control antibody (IC003R, open histogram).View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy (1, 2). Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human MUC16 that can vary from 1148 to 22152 amino acids (aa) in length and 200 - 5000 kDa in size (1, 2). The 22152 aa form contains ser/thr-rich N-terminal tandem repeats, 4 LRR (Leu-rich repeat) domains, 56 SEA (sea urchin sperm protein, enterokinase and agrin) domains, a transmembrane domain, and a 31 aa cytoplasmic domain that includes a tyrosine phosphorylation site (1-4). SEA domains are ~120 aa in length, contain conserved residues including potential O-glycosylation sites and a pair of cysteines, and are often found in transmembrane mucins (3). The protein produced by R&D Systems represents aa 13360-14347 of the full sequence and includes the last 6 SEA domains. It shares 68% aa identity with canine MUC16. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers (5). The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity (5-7). MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells (8). MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion (9, 10). MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy (10). In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome (11).
- Yin, B. W. T. K. O. Lloyd, 2001, J. Biol. Chem. 276:27371.
- Maeda, T. et al. (2004) J. Biol. Chem. 279:13174.
- Fendrick, J. L. et al. (1997) Tumour Biol. 18:278.
- Swissprot accession Q8WXI7.
- Kaneko, O. et al. (2009) J. Biol. Chem. 284:3739.
- Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
- Gubbels, J. A. A. et al. (2006) Mol. Cancer 5:50.
- Seelenmeyer, C. et al. (2003) J. Cell Sci. 116:1305.
- Patankar, M. S. et al. (2005) Gynecol. Oncol. 99:704.
- Belisle, J. A. et al. (2007) Immunology 122:418.
- Blalock, T. D. et al. (2007) Invest. Ophthalmol. Vis. Sci. 48:4509.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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