Nitric oxide (NO) is a pleiotropic signaling molecule implicated in diverse biological processes including inhibition of platelet aggregation, regulation of neurotransmission, vasodilation, immune responses, and inflammation. NO is synthesized from L-arginine and O2 by three nitric oxide synthase (NOS) enzymes termed endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). Each enzyme isoform is expressed in a variety of tissues and cell types. While eNOS and nNOS generally exhibit constitutive expression and are involved in physiological signaling and maintenance functions, iNOS expression is induced by inflammatory stimuli and is associated with both normal and pathological immune responses.
Human eNOS Quantikine ELISA Kit
R&D Systems | Catalog # DEN00
Discontinued Product
DEN00 has been discontinued.
View all eNOS products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Lysates (100 µL)
Sensitivity
25 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Lysates)
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Product Summary for Human eNOS Quantikine ELISA Kit
The Quantikine Human eNOS Immunoassay kit is a 4.5 hour solid phase ELISA designed to measure eNOS from endothelial cells. It contains E. coli-expressed recombinant human eNOS and antibodies raised against the recombinant enzyme. It has been shown to accurately quantitate recombinant human eNOS. Results obtained using natural human eNOS showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human eNOS.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Species
Human
Specificity
Natural and recombinant human eNOS
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 127 | 619 | 1280 | 129 | 621 | 1275 |
| Standard Deviation | 6.2 | 22.9 | 51.8 | 9.6 | 27.5 | 45.7 |
| CV% | 4.9 | 3.7 | 4.0 | 7.4 | 4.4 | 3.6 |
Linearity
To assess the linearity of the assay, five cell lysate samples containing high concentrations of eNOS were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: eNOS
Long Name
Endothelial Nitric Oxide Synthase
Alternate Names
NOS3
Gene Symbol
NOS3
Additional eNOS Products
Product Documents for Human eNOS Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human eNOS Quantikine ELISA Kit
For research use only
Related Research Areas
Citations for Human eNOS Quantikine ELISA Kit
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