Human Hrk Antibody

R&D Systems | Catalog # AF851

R&D Systems
Discontinued Product
AF851 has been discontinued. View all Hrk products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Hrk Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Hrk synthetic peptide
RTMWRRRARSR RAPAPGAALPC

Specificity

Detects human Hrk in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Hrk Antibody

Detection of Human Hrk antibody by Western Blot.

Detection of Human Hrk by Western Blot.

Western blot shows lysates ofE. coliexpressing recombinant human Hrk-green fluorescent protein fusion. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Hrk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF851) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Hrk was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human Hrk Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: E. coli expressing recombinant human Hrk-green fluorescent protein fusion

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hrk

Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL.

Long Name

Harakiri

Alternate Names

activator of apoptosis harakiri, activator of apoptosis Hrk, BCL2-interacting protein, BH3-interacting domain-containing protein 3, BID3, death protein 5, DP5, HARAKIRI, harakiri, BCL2 interacting protein (contains only BH3 domain), harakiri, BCL2-interacting protein (contains only BH3 domain), Neuronal death protein DP5

Entrez Gene IDs

8739 (Human)

Gene Symbol

HRK

Additional Hrk Products

Product Documents for Human Hrk Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Hrk Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human Hrk Antibody

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