Detection of Human Hrk by Western Blot.
Western blot shows lysates of E. coli expressing recombinant human Hrk-green fluorescent protein fusion. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Hrk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF851) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Hrk was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL.
Entrez Gene IDs:
activator of apoptosis harakiri; activator of apoptosis Hrk; BCL2-interacting protein; BH3-interacting domain-containing protein 3; BID3; death protein 5; DP5; HARAKIRI; harakiri, BCL2 interacting protein (contains only BH3 domain); harakiri, BCL2-interacting protein (contains only BH3 domain); Hrk; Neuronal death protein DP5
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