Interleukin 36 beta (IL-36 beta), also known as IL-1F8, FIL-1h and IL-1H2, is a member of the IL-1 family of proteins. IL-36 beta is reported to be actively secreted. Cells expressing IL-36 beta include resting and activated monocytes, and B cells. The receptor for IL-36 beta is thought to be a combination of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta activates pathways involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner.
Human IL-36 beta High Sensitivity Magnetic Luminex® Performance Assay
R&D Systems | Catalog # LBHS1099
Luminex High Performance Assays
Human IL-36 beta High Sensitivity Magnetic Luminex® Performance Assay is specifically designed for multiplex detection of human IL-36 beta/IL-1F8 with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.
This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.
Key Product Details
Panel
Human High Sensitivity Cytokine Panel B
Assay Type
Magnetic bead-based multiplex assay for the Luminex® platform
Format
1 x 96-well microplate and magnetic antibody-coated beads
Species
Human
Analytes Detected
IL-36 beta/IL-1F8
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Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human IL-36 beta/IL-1F8 with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Base Kit Contents
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in forty-three separate assays to assess precision between assays. |
||||||
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 43 | 43 | 43 |
| Mean (pg/mL) | 4.05 | 40.8 | 456 | 4.38 | 46.1 | 409 |
| Standard deviation | 0.109 | 0.803 | 10.2 | 0.618 | 5.16 | 48.1 |
| CV (%) | 2.7 | 2.0 | 2.2 | 14.1 | 11.2 | 11.8 |
Recovery
|
Samples containing and/or spiked with human IL-36B were evaluated for recovery. |
||||||
| Sample Type | Average % Recovery | Range | ||||
|---|---|---|---|---|---|---|
| Cell culture supernate | 110 | 100-115% | ||||
| Serum | 105 | 100-109% | ||||
| EDTA plasma | 102 | 93-113% | ||||
| Heparin plasma | 106 | 100-113% | ||||
Linearity
|
Samples containing and/or spiked with human IL-36B were serially diluted to evaluate assay linearity. |
|||||||||
| Cell culture supernate | Serum | EDTA plasma | Heparin plasma | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 100 | 96 | 94 | 92 | ||||
| Range (%) | 100-100 | 93-110 | 91-96 | 88-95 | |||||
| 1:4 | Average % of Expected | 101 | 99 | 84 | 81 | ||||
| Range (%) | 101-102 | 86-112 | 75-88 | 73-88 | |||||
| 1:8 | Average % of Expected | 105 | 99 | 80 | 80 | ||||
| Range (%) | 104-107 | 81-117 | 70-90 | 69-91 | |||||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human IL-36 beta High Sensitivity Magnetic Luminex® Performance Assay
Quantist software graph of signal intensity versus concentration for IL-36 beta/IL-1F8 in the Human High Sensitivity Cytokine B Luminex Panel
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-36 beta/IL-1F8
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Alternate Names
FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B
Gene Symbol
IL36B
Additional IL-36 beta/IL-1F8 Products
Product Documents for Human IL-36 beta High Sensitivity Magnetic Luminex® Performance Assay
Product Specific Notices for Human IL-36 beta High Sensitivity Magnetic Luminex® Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
Related Research Areas
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Associated Pathways
