MKI67 (also Ki-67) is a 350-400 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki-67 was originally recognized as an antigen associated with the monoclonal Ki-67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki-67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120 aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345 kDa in size and shows a deletion of aa 136-495, while a second isoform contains a 58 aa substitution for aa 1-513. Over aa 3120-3256, human Ki-67 shares 46% aa sequence identity with the mouse ortholog to Ki-67.
Human Ki67/MKI67 Alexa Fluor® 700-conjugated Antibody
R&D Systems | Catalog # IC7617N
Key Product Details
Species Reactivity
Applications
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Antibody Source
Product Specifications
Immunogen
Accession # P46013
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Host
Isotype
Applications for Human Ki67/MKI67 Alexa Fluor® 700-conjugated Antibody
Intracellular Staining by Flow Cytometry
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Reviewed Applications
Read 3 reviews rated 3.3 using IC7617N in the following applications:
Spectra Viewer
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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- Antigen Density Selector - Match fluorochrome brightness with antigen density
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Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ki67/MKI67
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Additional Ki67/MKI67 Products
Product Documents for Human Ki67/MKI67 Alexa Fluor® 700-conjugated Antibody
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Product Specific Notices for Human Ki67/MKI67 Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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Application: Flow CytometrySample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 08/05/2025
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Application: Flow CytometrySample Tested: Peripheral blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 12/29/2022
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Application: Flow CytometrySample Tested: Blood mononuclear cells (PBMCs)Species: HumanVerified Customer | Posted 12/29/2022
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