Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PAK6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PAK6
Met1-Arg137
Accession # Q9NQU5

Specificity

Detects endogenous human, mouse and rat PAK6 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PAK6 Antibody

Detection of Human/Mouse/Rat PAK6 antibody by Western Blot.

Detection of Human/Mouse/Rat PAK6 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK6 at approximately 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 3.

Applications for Human/Mouse/Rat PAK6 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PAK6

As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. PAK6 is expressed most highly in brain and testes, with lower levels in multiple tissues. Both MKK6 and p38 MAPK activate PAK6, suggesting a role for this kinase in the cellular stress response.

Long Name

p21/Cdc42/Rac1-activated Kinase 6

Alternate Names

EC 2.7.11, p21 protein (Cdc42/Rac)-activated kinase 6, p21(CDKN1A)-activated kinase 6, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK5EC 2.7.11.1, PAK-6, serine/threonine-protein kinase PAK 6

Entrez Gene IDs

56924 (Human); 214230 (Mouse); 296078 (Rat)

Gene Symbol

PAK6

UniProt

Q9NQU5

Additional PAK6 Products

Product Documents for Human/Mouse/Rat PAK6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PAK6 Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Human/Mouse/Rat PAK6 Antibody

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