Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell adÂhesion, and migration. Its expression is upregulatÂed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.
Human Osteopontin (OPN) Magnetic Luminex® Performance Assay
R&D Systems | Catalog # LHK1433
Luminex High Performance Assays
Human Osteopontin (OPN) Magnetic Luminex® Performance Assay is specifically designed for multiplex detection of human Osteopontin/OPN with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.
This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.
Key Product Details
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Assay Type
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Analytes Detected
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Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human Osteopontin/OPN with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Base Kit Contents
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
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Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in separate assays to assess precision between assays. |
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| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 72 | 70 | 72 |
| Mean (pg/mL) | 2702 | 17,630 | 121,750 | 2717 | 17,696 | 118,682 |
| Standard deviation | 59.6 | 232 | 3919 | 277 | 1031 | 11.549 |
| CV (%) | 2.2 | 1.3 | 3.2 | 10.2 | 5.8 | 9.7 |
Linearity
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Samples containing and/or spiked with human Osteopontin were serially diluted to evaluate assay linearity. |
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| Serum | EDTA plasma | Heparin plasma | Urine | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 106 | 106 | 96 | 97 | ||||
| Range (%) | 101-111 | 101-110 | 94-97 | 90-102 | |||||
| 1:4 | Average % of Expected | 108 | 102 | 96 | 97 | ||||
| Range (%) | 97-120 | 101-102 | 91-102 | 93-100 | |||||
| 1:8 | Average % of Expected | 110 | 103 | 73 | 102 | ||||
| Range (%) | 96-133 | 101-104 | 63-83 | 100-104 | |||||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human Osteopontin (OPN) Magnetic Luminex® Performance Assay
Quantist software graph of signal intensity versus concentration for Osteopontin/OPN in the Human Kidney Biomarker Luminex Panel
Preparation and Storage
Shipping
Stability & Storage
Background: Osteopontin/OPN
Long Name
Alternate Names
Gene Symbol
Additional Osteopontin/OPN Products
Product Documents for Human Osteopontin (OPN) Magnetic Luminex® Performance Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Osteopontin (OPN) Magnetic Luminex® Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
Citations for Human Osteopontin (OPN) Magnetic Luminex® Performance Assay
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