Human ROR gamma /RORC/NR1F3 Antibody

Catalog # Availability Size / Price Qty
MAB61092
MAB61092-SP
Detection of Human ROR gamma /RORC/NR1F3 by Western Blot.
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Human ROR gamma /RORC/NR1F3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROR gamma /RORC/NR1F3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ROR1, rhROR2, rhROR gamma t, or rhROR alpha is observed.
Source
Monoclonal Mouse IgG2B Clone # 732111
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ROR gamma /RORC/NR1F3
Met1-Gln121
Accession # P51449
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ROR?/RORC/NR1F3 antibody by Western Blot. View Larger

Detection of Human ROR gamma /RORC/NR1F3 by Western Blot. Western blot shows lysates of human Th1 cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ROR gamma/RORC/NR1F3 Monoclonal Antibody (Catalog # MAB61092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma/RORC/NR1F3 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR gamma/RORC/NR1F3

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑121 human ROR gamma shares 97% identity with mouse ROR gamma.

Long Name
Retinoic Acid-Related Orphan Receptor gamma
Entrez Gene IDs
6097 (Human)
Alternate Names
MGC129539; NR1F3; Nuclear receptor ROR gamma; Nuclear receptor ROR-gamma; Nuclear receptor RZR gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-Related Orphan Nuclear Receptor Variant 2; RAR-related orphan receptor C; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR gamma; RORC; RORG; RORGMGC129539; RZRG; RZRGRZR-GAMMA; TOR

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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