Detection of Human Th‑POK by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Th‑POK Monoclonal Antibody (Catalog # MAB6599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Th‑POK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Th-POK (T helper-inducing POZ/Kruppel-like factor; also hcKrox-a and zinc finger and BTB domain-containing protein 7B) is a 62-68 kDa member of the Krox family of transcription factors. It is expressed in immature T cells where it promotes commitment to the CD4+ T cell lineage. It is also expressed in fibroblasts and chondrocytes where it promotes type I and type II collagen secretion. Human Th-POK is 539 amino acids (aa) in length. It contains a BTB/POZ domain (aa 34-115) that mediates homo- and heterodimerization with hcKROX-b and -g, a Pro-rich segment (aa 181-196), and four C-terminal C2H2-type zinc finger regions (aa 346-454). There is one variant that shows a 34 aa N-terminal extension. Over aa 78-172, human Th-POK is 97% aa identical to mouse Th-POK.
T-helper-inducing POZ/Krueppel-like Factor
Entrez Gene IDs:
c-Krox; hcKroxc-Krox; Krueppel-related zinc finger protein cKrox; kruppel-related zinc finger protein hcKrox; T-helper-inducing POZ/Krueppel-like factor; ThPOK; Th-POK; ZBTB15; ZBTB15zinc finger protein 67 homolog (mouse); ZBTB7B; ZFP67; zfp-67; zinc finger and BTB domain containing 15; zinc finger and BTB domain containing 7B; Zinc finger and BTB domain-containing protein 15; zinc finger and BTB domain-containing protein 7B; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th-POK; ZNF857B; ZNF857BDKFZp686G01254
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