Human Th-POK Antibody

Name: Human Th-POK Antibody
Brand: R&D Systems
SKU: MAB6599

Catalog #: MAB6599 Datasheet / COA / SDS

Discontinued Product

MAB6599 has been discontinued.

View all Th-POK products.

Detection of Human Th‑POK by Western Blot.

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All Th-POK Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Th-POK Antibody Summary

Species Reactivity

Human

Specificity

Detects human Th-POK in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 615819

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli derived human Th-POK
Gly78-Ser172
Accession # Q64321

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Th‑POK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Th-POK Monoclonal Antibody (Catalog # MAB6599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Th-POK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Th-POK

Th-POK (T helper-inducing POZ/Kruppel-like factor; also hcKrox-a and zinc finger and BTB domain-containing protein 7B) is a 62-68 kDa member of the Krox family of transcription factors. It is expressed in immature T cells where it promotes commitment to the CD4⁺ T cell lineage. It is also expressed in fibroblasts and chondrocytes where it promotes type I and type II collagen secretion. Human Th-POK is 539 amino acids (aa) in length. It contains a BTB/POZ domain (aa 34-115) that mediates homo- and heterodimerization with hcKROX-b and -g, a Pro-rich segment (aa 181-196), and four C-terminal C2H2-type zinc finger regions (aa 346-454). There is one variant that shows a 34 aa N-terminal extension. Over aa 78-172, human Th-POK is 97% aa identical to mouse Th-POK.

Long Name

T-helper-inducing POZ/Krueppel-like Factor

Entrez Gene IDs

51043 (Human)

Alternate Names

c-Krox; hcKroxc-Krox; Krueppel-related zinc finger protein cKrox; kruppel-related zinc finger protein hcKrox; T-helper-inducing POZ/Krueppel-like factor; ThPOK; Th-POK; ZBTB15; ZBTB15zinc finger protein 67 homolog (mouse); ZBTB7B; ZFP67; zfp-67; zinc finger and BTB domain containing 15; zinc finger and BTB domain containing 7B; Zinc finger and BTB domain-containing protein 15; zinc finger and BTB domain-containing protein 7B; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th-POK; ZNF857B; ZNF857BDKFZp686G01254