Human Th‑POK Antibody

R&D Systems | Catalog # MAB6599

R&D Systems
Discontinued Product
MAB6599 has been discontinued. View all Th-POK products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 615819
View all Th-POK Primary Antibodies »

Product Specifications

Immunogen

E. coli derived human Th-POK
Gly78-Ser172
Accession # Q64321

Specificity

Detects human Th-POK in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Th‑POK Antibody

Detection of Human Th-POK antibody by Western Blot.

Detection of Human Th‑POK by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Th-POK Monoclonal Antibody (Catalog # MAB6599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Th-POK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Th‑POK Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Th-POK

Th-POK (T helper-inducing POZ/Kruppel-like factor; also hcKrox-a and zinc finger and BTB domain-containing protein 7B) is a 62-68 kDa member of the Krox family of transcription factors. It is expressed in immature T cells where it promotes commitment to the CD4+ T cell lineage. It is also expressed in fibroblasts and chondrocytes where it promotes type I and type II collagen secretion. Human Th-POK is 539 amino acids (aa) in length. It contains a BTB/POZ domain (aa 34-115) that mediates homo- and heterodimerization with hcKROX-b and -g, a Pro-rich segment (aa 181-196), and four C-terminal C2H2-type zinc finger regions (aa 346-454). There is one variant that shows a 34 aa N-terminal extension. Over aa 78-172, human Th-POK is 97% aa identical to mouse Th-POK. 

Long Name

T-helper-inducing POZ/Krueppel-like Factor

Alternate Names

c-Krox, ThPOK, ZBTB15, ZBTB7B, ZFP67, ZNF857B

Entrez Gene IDs

51043 (Human)

Gene Symbol

ZBTB7B

UniProt

Q64321

Additional Th-POK Products

Product Documents for Human Th‑POK Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Th‑POK Antibody

For research use only

Related Research Areas

Natural Killer T (NKT) Cells

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