Recombinant Human Indian Hedgehog/Ihh C28II Protein

Novus Biologicals | Catalog # NBP2-35012

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Key Product Details

Source

E. coli

Accession Number

Q14623

Applications

Bioactivity, SDS-PAGE
View all Indian Hedgehog/Ihh Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 176 amino acids corresponding to Indian Hedgehog/Ihh Source: E. coli

Uniprot ID: Q14623

Amino Acid Sequence: IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Indian Hedgehog/Ihh as determined by LAL method.

Predicted Molecular Mass

19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Indian Hedgehog Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ug/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-35012
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, 0.02% Tween-20, pH 7.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Indian Hedgehog/Ihh

IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in adult kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with murine.

Alternate Names

BDA1, HHG2, Ihh

Gene Symbol

IHH

UniProt

Q14623 (Human)

Additional Indian Hedgehog/Ihh Products

Product Documents for Recombinant Human Indian Hedgehog/Ihh C28II Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Indian Hedgehog/Ihh C28II Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human Indian Hedgehog/Ihh C28II Protein

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