LIME Antibody (LIME-06) - BSA Free

Novus Biologicals | Catalog # NBP1-44945

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow (Intracellular), Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LIME-06

Format

BSA Free
View all LIME Primary Antibodies »

Product Specifications

Immunogen

Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.

Specificity

The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIME Antibody (LIME-06) - BSA Free

Immunoprecipitation: LIME Antibody (LIME-06) - BSA Free [NBP1-44945]

Immunoprecipitation: LIME Antibody (LIME-06) - BSA Free [NBP1-44945]

Immunoprecipitation: LIME Antibody (LIME-06) [NBP1-44945] - Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. NB500-521) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).

Applications for LIME Antibody (LIME-06) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

8-12 ug/ml

Immunoprecipitation

1:50

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LIME

LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]).[supplied by OMIM]

Alternate Names

dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067

Gene Symbol

LIME1

Additional LIME Products

Product Documents for LIME Antibody (LIME-06) - BSA Free

Certificate of Analysis

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Product Specific Notices for LIME Antibody (LIME-06) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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