LinkAble™ APC Antibody Conjugation Kits
R&D Systems | Catalog # LNKAPC
Key Product Details
Applications
Assay Type
Conjugate
Product Summary for LinkAble™ APC Antibody Conjugation Kits
LinkAble™ APC Antibody Conjugation Kits enable rapid, in house antibody labeling and conjugation to allophycocyanin (APC) through primary amine groups (e.g. lysines). Designed for straightforward, in-house use, the APC conjugation kit supports reliable, reproducible conjugation with minimal hands-on time and no requirement for specialized expertise or additional purification steps. Conjugates are ready to use in approximately two hours, supporting efficient evaluation and comparison of antibodies in downstream assays.
The kits are optimized for single-use reactions and are also available in multipack formats to support higher-throughput or repeat experimental workflows.
Key Benefits
- Ease & Efficiency: Streamlined protocols ensure a simple, fast, and reliable conjugation process. The kit requires only 30 seconds of hands-on time and produces conjugates that are ready to use in 2 hours, without any loss of antibody associated with buffer exchange and purification.
- Reproducibility: Optimized reagents deliver consistent, high-quality results. The kit is available in 25 µg, 100 µg, and 1 mg antibody input sizes, which have been optimized to perform identically across all three sizes, allowing scaling of conjugation reactions.
- Streamlined & Optimized Usability: The ready-to-use format eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. An intuitive, user friendly design includes screw cap vials that can be easily centrifuged to recover the sample.
Starting Mass & Volume of Antibody
The kit produces high quality conjugates using IgG antibodies with a concentration of 250 µg/mL or greater. For optimal results, it is recommended to carry out the conjugation reaction with antibody concentration at 1 mg/mL.
| Reaction Size | Minimum (μg) | Optimal (μg) | Maximum (μg) |
|---|---|---|---|
| 25 μg | 10 | 25 | 100 |
| 100 μg | 25 | 100 | 400 |
| 1000 μg | 250 | 1000 | 4000 |
Technical Hints & Limitations
- Although it is recommended to use a purified antibody in a suitable buffer (e.g. PBS) and free from additives, the LinkAble™ APC Antibody Conjugation Kit produces high quality conjugates in the presence of some buffers/additives.
Note: The kit is not compatible with certain components, as described in the table below.
| Components | Compatible |
|---|---|
| Amine-free buffer (MES, PBS, HEPES, bicarb, borate) | Yes |
| Tris | ≤ 100 mM |
| Sugars (trehalose / sucrose) | Yes |
| EDTA | Yes |
| Proclin | Yes |
| Sodium azide | No |
| BSA | ≤ 0.1% |
| Glycerol | ≤ 10% |
| Amine-containing reagents (glycine, arginine) | No |
- If multiple, non-optimal parameters are used in the conjugation reaction (concentration, additives, etc.) poor quality conjugates may result.
- Centrifuge all vials before resuspending to ensure all contents are at the bottom of the vials.
- In each step, gently pipette the sample up and down to mix.
- Purification of the conjugated antibody is not necessary, though a user may choose to do so.
Scientific Data Images for LinkAble™ APC Antibody Conjugation Kits
Enhanced Performance of CD3-APC Conjugates Prepared Using LinkAble™ APC Antibody Conjugation Kit Versus Other Supplier Kit
Mouse anti-CD3 (MAB100) was conjugated using LinkAble™ APC Antibody Conjugation Kit and an APC conjugation kit from another supplier. Human peripheral blood lymphocytes were stained following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.
High Reproducibility of CD3-APC Conjugates Made Using LinkAble™ APC Antibody Conjugation Kits
Three Mouse anti-CD3 (MAB100) conjugates were prepared using LinkAble™ APC Antibody Conjugation Kits and then used to stain human peripheral blood lymphocytes following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.
Consistent Batch-to-Batch Performance of CD3-APC Conjugates Made Using LinkAble™ APC Antibody Conjugation Kits
Mouse anti-CD3 (MAB100) APC conjugates were prepared using three independent batches of LinkAble™ APC Antibody Conjugation Kits and then used to stain human peripheral blood lymphocytes following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.
Consistent CD3-APC Conjugate Performance Across Reaction Sizes Made Using LinkAble™ APC Antibody Conjugation Kits
Mouse anti-CD3 (MAB100) was conjugated using LinkAble™ APC Antibody Conjugation Kits (25 µg, 100 µg, and 1000 µg sizes) and then used to stain human peripheral blood lymphocytes following the Staining Membrane-associated Proteins protocol and analyzed by flow cytometry.Kit Contents for LinkAble™ APC Antibody Conjugation Kits
- 10X Buffer: 200 μL (3 x 200 µL for 5 x 1 mg kit)
- Reagent A: 1 vial (Lyophilized; x3 or x5 for multi-pack kits)
- Reagent B: 1 vial (Lyophilized; x3 or x5 for multi-pack kits)
Other Materials Required
Antibody to be conjugated. Refer to Technical Hints and Limitations for more information on the antibody requirements.
Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for LinkAble™ APC Antibody Conjugation Kits
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for LinkAble™ APC Antibody Conjugation Kits
LinkAbleTM is a trademark of Bio-Techne.
Wear appropriate protective gloves, clothing, and eye/face protection. Wash hands thoroughly after handling. Consult the Safety Data Sheet (SDS) on our website prior to use. Review SDS information for all kit components, including the borate buffer.
For research use only
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Protocols
View specific protocols for LinkAble™ APC Antibody Conjugation Kits (LNKAPC):
General Protocol
- Add sufficient volume of 10X Reaction Buffer to the antibody solution so the final Reaction Buffer concentration is 1X.
- Transfer the antibody solution in 1X Reaction Buffer to the Reagent A tube and gently pipette up and down to dissolve.
- Incubate the tube at room temperature for 60 minutes in the dark.
- Transfer the antibody solution to the Reagent B tube and gently pipette up and down to dissolve.
- Incubate the tube at room temperature for 60 minutes in the dark.
- The conjugated antibody is now ready to use.
Note: No conjugation clean-up is required before the labeled antibody can be used.
Conjugate Storage
The conjugated antibody should be stored at 4 °C.
Troubleshooting
The efficiency of the conjugation reaction means there are small quantities of materials in each of the vials. We recommend centrifuging before starting the conjugation to ensure all material is at the bottom of the vial. For additional troubleshooting assistance or any other questions, contact our technical support team.
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