Chemical Name: 2-[[4-(Cyclopropylcarbonyl)-1-piperazinyl]methyl]-1,2-benzisothiazol-3(2H)-one
Biological ActivityLOC14 is a high affinity protein disulfide isomerase (PDI) modulator (Kd = 62 nM). Forces PDI to adopt an oxidized conformation and suppresses its activity. Neuroprotective.
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Small molecule-induced oxidation of protein disulfide isomerase is neuroprotective.
Kaplan et al.
Citation for LOC14
The citations listed below are publications that use Tocris products. Selected citations for LOC14 include:
1 Citation: Showing 1 - 1
Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics.
Authors: Chamberlain Et al.
Redox Biol 2019;22:101129
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Washed platelets (2 x 10ˆ7 platelets/mL) were incubated in absence or presence of LOC14 (0.1, 1 and 10 µM) for 10 minutes at 37°C, then 300 µL of the solution was dispensed on a fibrinogen (100 µg/mL)-coated coverslip for 45 minutes at 37°C. Non-adhered platelets were removed and the coverslip washed three times with PBS. Adhered platelets were fixed using 0.2% paraformaldehyde for 10 minutes. This solution was then removed and coverslips washed three times with PBS before the addition of 0.1% (v/v) Triton-X to permeabilise the cells. After removal of Triton-X and further washes using PBS, platelets were stained with Alexa Fluor 488-conjugated phalloidin (1:1000 v/v) for 1 hour in the dark at room temperature. Coverslips were mounted onto microscope glass slides and imaged using a 100x oil immersion lens on a Nikon A1-R Confocal microscope.