Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein

Novus Biologicals | Catalog # NBP1-99017

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-386 of Human Methionine Aminopeptidase 1/METAP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWAT HKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSE SEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMI KPGVTTEEID HAVHLACIAR NCYPSPLNYY NFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIE PMICEGGWQD ETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

45.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein

Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein

SDS-Page: Recombinant Human Methionine Aminopeptidase 1/METAP1 Protein [NBP1-99017] -

SDS-Page: Recombinant Human Methionine Aminopeptidase 1/METAP1 Protein [NBP1-99017] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-99017
Formulation 20 mM Tris-HCl buffer (pH 8.0), 3mM DTT, 40% glycerol, 200 mM NaCl, 0.1 mM PMSF
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionine Aminopeptidase 1/METAP1

METAP1 (Methionine aminopeptidase 1) belongs to the peptidase M24A family. This protein removes the amino-terminal methionine from nascent proteins. It required for normal progression through the cell cycle. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant human METAP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

MAP1, MetAP1

Gene Symbol

METAP1

Additional Methionine Aminopeptidase 1/METAP1 Products

Product Documents for Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

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