Recombinant Rat MIF Protein

Novus Biologicals | Catalog # NBP2-35276

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Key Product Details

Source

E. coli

Applications

SDS-PAGE
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Product Specifications

Description

A single non-glycosylated polypeptide chain containing 115 amino acids corresponding to MIF Source: E. coli

Uniprot ID: P30904

Amino Acid Sequence: MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of MIF as determined by LAL method.

Predicted Molecular Mass

12.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Test in process

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat MIF Protein

SDS-PAGE: Recombinant Rat MIF Protein [NBP2-35276]

SDS-PAGE: Recombinant Rat MIF Protein [NBP2-35276]

SDS-Page: Rat MIF Protein [NBP2-35276]

Formulation, Preparation, and Storage

NBP2-35276
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose.
Preservative Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIF

Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively.

Long Name

Macrophage Migration Inhibitory Factor

Alternate Names

EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase

Gene Symbol

MIF

Additional MIF Products

Product Documents for Recombinant Rat MIF Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat MIF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Rat MIF Protein

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FAQs for Recombinant Rat MIF Protein

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  • Q: Could we use this protein for in vivo assay and cell culture (supplements)?

    A: This is a recombinant protein expressed in E. coli. Therefore it is not properly glycosylated, if at all. However, it has been tested for the levels of the endotoxin: Less than 1 EU/ug of endotoxin as determined by LAL method. In this case, this may be used in the live culture (in vivo), without eliciting any unwanted side effects.

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