MIS12 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2358

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MIS12 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIS12 Antibody - BSA Free

Immunoprecipitation: MIS12 Antibody [NB100-2358]

Immunoprecipitation: MIS12 Antibody [NB100-2358]

Immunoprecipitation: MIS12 Antibody [NB100-2358] - Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody NB100-2358 used for IP at 3 ug per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody NB100-2359. For blotting immunoprecipitated MIS12, NB100-2359 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MIS12 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Immunoprecipitation Not recommended for Western Blot. Use rabbit anti-MIS12 antibody Cat. No. NB 100-2359.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MIS12

MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly.

Alternate Names

2510025F08Rik, hMIS12, homolog of yeast Mis12, KNTC2AP, MGC2488, MIS12 homolog, MIS12, MIND kinetochore complex component, homolog (S. pombe), MIS12, MIND kinetochore complex component, homolog (yeast), MTW1, protein MIS12 homolog

Entrez Gene IDs

79003 (Human)

Gene Symbol

MIS12

UniProt

NP_076944.1 (Human)

Additional MIS12 Products

Product Documents for MIS12 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MIS12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MIS12 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review MIS12 Antibody - BSA Free and earn rewards!

Have you used MIS12 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies