Endocan, also known as Endothelial Cell-specific Molecule-1 (ESM-1) and IGFBP-rp6, is a secreted cysteine-rich dermatan sulfate proteoglycan. It is primarily expressed by endothelial cells within the vascular network of the kidney and in the alveolar walls of the lung. Endocan expression is upregulated by TNF-alpha, IL-1 beta and LPS, and downregulated by IFN-gamma. Endocan binds LFA-1 on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation. Elevated circulating Endocan levels have been reported in patients with lung cancer and severe sepsis.
Discontinued Product
MEND00 has been discontinued.
View all Endocan/ESM-1 products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity
21.3 pg/mL
Assay Range
46.9-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse Endocan Quantikine ELISA Kit
The Quantikine Mouse Endocan immunoassay is a 4.5 hour solid-phase ELISA designed to measure Endocan in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse Endocan and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Endocan. Results obtained using natural mouse Endocan showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Endocan.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Species
Mouse
Specificity
Natural and recombinant mouse Endocan
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 143 | 398 | 1107 | 137 | 373 | 1097 |
| Standard Deviation | 14.1 | 25.3 | 70.7 | 13.1 | 23.2 | 78.4 |
| CV% | 9.8 | 6.4 | 6.4 | 9.6 | 6.2 | 7.1 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 154 | 515 | 1439 | 206 | 517 | 1406 |
| Standard Deviation | 14.8 | 25.4 | 86 | 20.8 | 39.2 | 99.8 |
| CV% | 9.6 | 4.9 | 6 | 10.1 | 7.6 | 7.1 |
Recovery for Mouse Endocan Quantikine ELISA Kit
The recovery of mouse Endocan spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 99 | 91-120 |
| EDTA Plasma (n=4) | 92 | 81-119 |
| Heparin Plasma (n=4) | 108 | 89-119 |
| Serum (n=4) | 92 | 81-117 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Endocan in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Endocan/ESM-1
Alternate Names
ESM-1, ESM1, IGFBP-rp6
Gene Symbol
ESM1
Additional Endocan/ESM-1 Products
Product Documents for Mouse Endocan Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Endocan Quantikine ELISA Kit
For research use only
Related Research Areas
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