Mouse Semaphorin 3A Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB109031N Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB109031N-100UG
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Mouse Semaphorin 3A Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 3A in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG1 Clone # 1040525
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived mouse Semaphorin 3A protein
Asn21-Lys747
Accession # O08665
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was bEnd.3 mouse brain endothelial cell line
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 3A

Semaphorin 3A (Sema3A; previously sem D, sema III or collapsin) is one of six Class 3 secreted semaphorins which share ~40-50% amino acid (aa) identity
(1-3). Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development (2, 3). The 772 aa mouse Sema3C contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a PSI domain, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (3, 4). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (5, 6). The 95 kDa mature mouse Sema3A shares at least 95% aa identity with human, rat, equine and canine Sema3A, and 90% and 86% aa identity with chick and zebrafish Sema3A, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (3). Sema3A signaling is transduced by plexin A1-4, indirectly via neuropilin-1 (3). Sema3A activity is mediated by small GTPases that influence actin rearrangement and integrin activity (7-9). It is important in developmental organization of central and peripheral nerves, including those in heart, lung, kidneys, bones, teeth, and visual and olfactory systems (1, 2, 10, 11). Gradients of Sema3A repel axons, but attract dendrites (11, 12). Sema3A affect vasculogenesis by inhibiting integrin function and, with Sema3F, promoting apoptosis of endothelial cells (3, 9, 12). It is thought to suppress cancer-related angiogenesis (3). In the immune system, Sema3A influences T cell proliferation, migration, response to activation, and interactions with dendritic cells (7, 13). It negatively regulates platelet activation (14). Expression of Sema3A in relevant parts of the nervous system may be increased in Alzheimer’s disease, multiple sclerosis, ischemia and schizophrenia (2).

References
  1. Puschel, A.W. et al. (1995) Neuron 14:941.
  2. Roth, L. et al. (2009) Cell. Mol. Life Sci. 66:649.
  3. Neufeld, G and O. Kessler (2008) Nat. Rev. Cancer 8:632.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  6. Klosterman, A. et al. (1998) J. Biol. Sci. 273:7326.
  7. Lepelletier, Y. et al. (2006) Eur. J. Immunol. 36:1782.
  8. Schlomann, U. et al. (2009) J. Cell Sci. 122:2034.
  9. Serini, G. et al. (2003) Nature 424:391.
  10. Ieda, M. et al. (2007) Nat. Med. 13:604.
  11. Chen, G. et al. (2008) Nat. Neurosci. 11:36.
  12. Guttmann-Raviv, N. et al. (2007) J. Biol Chem. 282:26294.
  13. Lepelletier, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5545.
  14. Kashiwagi, H. et al. (2005) Blood 106:913.
Entrez Gene IDs
10371 (Human); 20346 (Mouse); 29751 (Rat); 100620398 (Porcine); 102126862 (Cynomolgus Monkey)
Alternate Names
(semaphorin) 3A; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema III; SEMA1; Sema3A; SEMAD; SEMAIII; SEMAL; Semaphorin 3A; semaphorin D; Semaphorin III; semaphorin L; semaphorin-3A; SemD

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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