NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-25286

Novus Biologicals
100% Guarantee

Product Specifications

Kit Type

Assay Kit (Colorimetric)

Species

Human

Description

  • SEAP is secreted into the media so no cell lysis is required for the detection of its enzymatic activity.
  • A secreted indicator function permits multiple kinetics experiments using only one culture through sequential sampling of the medium, and also allows the cells to be used for other purposes, such as RNA extraction, Western blot analysis, and other assays
  • SEAP is stable in serum, allowing it to be used as a reporter assay in vivo.
  • SEAP catalyzes the hydrolysis of pNitrophenyl phosphate (PNPP) producing a yellow product that can be read in a spectrophotometer or ELISA reader at 405 nm.

    • Scientific Data Images for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    Enzyme Activity: NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) [NBP2-25286]

    Enzyme Activity: NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) [NBP2-25286]

    Enzyme Activity: NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) [NBP2-25286] - Standard curve for the SEAP protein provided in the NF-KB SEAPorter(TM) Assay Kit. A serial dilution of SEAP protein was added to a 96-well microtiter plate and the standard curve was generated according to the Kit assay protocol.

    Kit Contents for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    • 10X PNPP Buffer (4 mL)
    • 10X Sample Dilution Buffer (10 mL)
    • 96-well Microplate (4 EA)
    • Microplate seal (4 EA)
    • pCMV/SEAP plasmid at 0.5 ug/uL (2 x 10 ug)
    • pNF-kappa B/SEAP plasmid at 0.5 ug/uL (2 x 10 ug)
    • PNPP Substrate (8 x 5 mg)
    • SEAP Standard (200 ug/mL)

    Preparation and Storage

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    Stability & Storage

    Storage of components varies. See protocol for specific instructions.

    Background: NFkB Secreted Alkaline Phosphatase Reporter

    Placental alkaline phosphatase (PLAP) is one of the most stable isoenzymes, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of PLAP is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is designed by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 (1): 10 (1988). This mutation converts the membrane-bound PLAP protein into the secreted protein SEAP. As a major transcription factor NF-KB plays a key role in regulating genes responsible for the innate and adaptive immune responses. In unstimulated cells, the NF-KB dimers are held in cytoplasm by IKBs that masks the nuclear localization signals of NF-KB. Upon cell stimulation, which leads to IKB degradation, NF-KB quickly translocates to the nucleus and activates various genes that have DNA-binding sites for NF-KB. Our NF-KB SEAPORTER Assay Kit is designed to measure NF-KB activation using SEAP protein secreted to the culture media as a read-out.

    Alternate Names

    SEAP assay

    Additional NFkB Secreted Alkaline Phosphatase Reporter Products

    Product Documents for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

    Citations for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    Customer Reviews for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    There are currently no reviews for this product. Be the first to review NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) and earn rewards!

    Have you used NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)?

    Submit a review and receive an Amazon gift card!

    $25/€18/£15/$25CAN/¥2500 Yen for a review with an image

    $10/€7/£6/$10CAN/¥1110 Yen for a review without an image

    Submit a review
    Amazon Gift Card

    Protocols

    View specific protocols for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) (NBP2-25286):

    NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric):
    Product and Contents
    Product Name Nf-kB SEAPorter Assay Kit, SEAPorter Assay Kit
    Catalog No. NBP2-25286

    Contents p-Nitrophenyl Phosphate (KC-103), Secreated Placental Alkaline Phosphatase (IMK-515-1), SEAP Plasmids (IMK-515-3, IMK-515-4), Sodium Azide (Sample Dilution Buffer, IMK-515-6)
    CAS#: 33013-2 (pNPP, KC-103)
    CAS#: None (SEAP, IMK-515-1)
    CAS#: None (SEAP Plasmids IMK-515-3, IMK515-4)
    CAS# 26628-22-8 (10X Sample Dilution Buffer with 0.05% Sodium Azide, IMK-515-6)
    p-Nitrophenyl Phosphate (pNPP) KC-103

    Hazardous Information
    Eye: Causes eye irritation.
    Skin: Causes skin irritation. May be harmful if absorbed through skin.
    Ingestion: May be harmful if swallowed. Call a physician
    Inhalation: May causes respiratory tract irritation. May be harmful if inhaled.
    Symptoms of Exposure: Irritation

    Handling
    Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.

    Storage
    Store at -20 degrees C. Store in a tightly closed container. Light and moisture sensitive. Stable under recommended storage conditions

    Personal Protection
    Wear appropriate protective clothing. Protect the skin by handling with gloves. Protect eyes by wearing face shield and safety glasses. Wash hands immediately before and after use.

    First Aid Measure
    Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Consult a physician.
    Skin: Flush skin with excess amounts of water for at least 15 minutes. Consult a physician
    Ingestion: Toxic material. If swallowed, get medical aid immediately. Only induce vomiting if directed to do so by medical personnel. Never give anything by mouth to an unconscious person.
    Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. SPEED IS ESSENTIAL, OBTAIN MEDICAL AID IMMEDIATELY. Do not use mouth-to-mouth resuscitation if victim ingested or enhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device.
    Notes: Consult a physician. Show this safety data sheet to the physician. Move away from the dangerous area.

    Secreted Alkaline Phosphatase
    Hazardous Information
    Eye: Causes eye irritation.
    Skin: Causes skin irritation. May be harmful if absorbed through skin.
    Ingestion: May be harmful if swallowed. Call a physician
    Inhalation: May causes respiratory tract irritation. May be harmful if inhaled.
    Symptoms of Exposure: Irritation

    Handling
    Handle as if capable of transmitting infectious agents. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.

    Storage
    Store at -20 degrees C. Store in a tightly closed container. Light and moisture sensitive. Stable under recommended storage conditions

    Personal Protection
    Wear appropriate protective clothing. Protect the skin by handling with gloves. Protect eyes by wearing face shield and safety glasses. Wash hands immediately before and after use.

    First Aid Measure
    Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Consult a physician.
    Skin: Flush skin with excess amounts of water for at least 15 minutes. Consult a physician
    Ingestion: POISON material. If swallowed, get medical aid immediately. Only induce vomiting if directed to do so by medical personnel. Never give anything by mouth to an unconscious person.
    Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. SPEED IS ESSENTIAL, OBTAIN MEDICAL AID IMMEDIATELY. Do not use mouth-to-mouth resuscitation if victim ingested or enhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device.
    Notes: Consult a physician. Show this safety data sheet to the physician. Move away from the dangerous area.

    SEAP Plasmids
    Hazardous Information
    Eye: Causes eye irritation.
    Skin: Causes skin irritation. May be harmful if absorbed through skin.
    Ingestion: May be harmful if swallowed. Call a physician
    Inhalation: May causes respiratory tract irritation. May be harmful if inhaled.
    Symptoms of Exposure: Irritation

    Handling
    Handle as if capable of transmitting infectious agents. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.

    Storage
    Store at -20 degrees C. Store in a tightly closed container. Light and moisture sensitive. Stable under recommended storage conditions

    Personal Protection
    Wear appropriate protective clothing. Protect the skin by handling with gloves. Protect eyes by wearing face shield and safety glasses. Wash hands immediately before and after use.

    First Aid Measure
    Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Consult a physician.
    Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. SPEEDY ACTION IS CRITICAL!
    Ingestion: POISON material. If swallowed, get medical aid immediately. Only induce vomiting if directed to do so by medical personnel. Never give anything by mouth to an unconscious person.
    Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. SPEED IS ESSENTIAL, OBTAIN MEDICAL AID IMMEDIATELY. Do not use mouth-to-mouth resuscitation if victim ingested or enhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device.
    Notes: Consult a physician. Show this safety data sheet to the physician. Move away from the dangerous area.

    10X Sample Dilution Buffer with 0.05% Sodium Azide

    Hazardous Information
    Eye: Causes eye irritation.
    Skin: Causes skin irritation. May be harmful if absorbed through skin.
    Ingestion: May be fatal if swallowed.
    Inhalation: May causes respiratory tract irritation. May be harmful if inhaled.
    Symptoms of Exposure: Irritation

    Handling
    Use with adequate ventilation. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Avoid ingestion and inhalation.

    Storage
    Store at 4 degrees C. Store in a tightly closed container. Light and moisture sensitive. Stable under recommended storage conditions

    Personal Protection
    Wear appropriate protective clothing. Protect the skin by handling with gloves. Protect eyes by wearing face shield and safety glasses. Wash hands immediately before and after use.

    First Aid Measure
    Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Consult a physician.
    Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. SPEEDY ACTION IS CRITICAL!
    Ingestion: POISON material. If swallowed, get medical aid immediately. Only induce vomiting if directed to do so by medical personnel. Never give anything by mouth to an unconscious person.
    Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. SPEED IS ESSENTIAL, OBTAIN MEDICAL AID IMMEDIATELY. Do not use mouth-to-mouth resuscitation if victim ingested or enhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device.
    Notes: Consult a physician. Show this safety data sheet to the physician. Move away from the dangerous area.

    FAQs for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric)

    Showing  1 - 1 of 1 FAQ Showing All

    • Q: Could you send me the protocol for NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Cat # NBP2-25286)?

      A: Please find the following link to our protocol for NBP2-25286: NBP2-25286 protocol

    Showing  1 - 1 of 1 FAQ Showing All
    View all FAQs for Activity Assays