Recombinant Mouse Persephin Protein

Novus Biologicals | Catalog # NBP2-35118

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

O70300

Applications

Bioactivity, SDS-PAGE
View all Persephin Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimer of two 96 amino acid polypeptide chains corresponding to Persephin Source: E. coli

Uniprot ID: O70300

Amino Acid Sequence: ALAGSCRLWS LTLPVAELGL GYASEEKVIF RYCAGSCPQE ARTQHSLVLA RLRGRGRAHG RPCCQPTSYA DVTFLDDQHH WQQLPQLSAA ACGCGG

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Persephin as determined by LAL method.

Predicted Molecular Mass

20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Persephin Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-35118
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 30 % ACN, 0.1 % TFA, 150 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Persephin

Persephin belongs to the GDNF ligand subfamily of the TGF-beta superfamily. It promotes the survival and growth of central dopaminergic and motor neurons, and also is involved in kidney development. But persephin does not support survival of peripheral neurons.

Alternate Names

PSPN

Gene Symbol

PSPN

UniProt

O70300

Additional Persephin Products

Product Documents for Recombinant Mouse Persephin Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Persephin Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family

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