PF 477736

Tocris Bioscience | Catalog # 4277

Selective Chk1 inhibitor
Tocris Bioscience
Discontinued Product
4277 has been discontinued. View all Checkpoint Kinase Inhibitors products.

Key Product Details

Description

Selective Chk1 inhibitor

Alternative Names

PF-0044736
View all Checkpoint Kinase Inhibitors »

Product Description

PF 477736 is a selective checkpoint kinase 1 (Chk1) inhibitor (Ki values are 0.49 and 47 nM for Chk1 and Chk2 respectively). Abrogates cell cycle arrest at S and G2-M checkpoints; sensitizes cells to DNA damage. Enhances docetaxel (Cat. No. 4056) efficacy in tumor cells and xenografts.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

External Portal Information

Chemicalprobes.org

is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of PF 477736 is reviewed on the chemical probes website.

Product Specifications for PF 477736

Molecular Weight

419.48

Formula

C22H25N7O2

Storage

Store at +4°C

Purity

≥99% (HPLC)

Chemical Name

(R)-α-Amino-N-[5,6-dihydro-2-(1-methyl-1H-pyrazol-4-yl)-6-oxo-1H-pyrrolo[4,3,2-ef][2,3]benzodiazepin-8-yl]-cyclohexaneacetamide

CAS Number

952021-60-2

PubChem ID

16750408

InChI Key

YFNWWNRZJGMDBR-LJQANCHMSA-N

SMILES

O=C([C@H](N)C4CCCCC4)NC1=CC3=C(C(C=NNC3=O)=C(C5=CN(C)N=C5)N2)C2=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 31.46 75

Preparing Stock Solutions for PF 477736

The following data is based on the product molecular weight 419.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.18 mL 15.89 mL 31.79 mL
3.75 mM 0.64 mL 3.18 mL 6.36 mL
7.5 mM 0.32 mL 1.59 mL 3.18 mL
37.5 mM 0.06 mL 0.32 mL 0.64 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 12 publications citing the usage of this product.

Product Documents for PF 477736

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PF 477736

For research use only

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