Specific for the ~95 kDa Dynamin protein phosphorylated at S778 in Western blots. Labels the purified protein phosphorylated in vitro by Cdk5 but not by PKC. Does not cross react with other purified substrates of Cdk5 (e.g. Amphiphysin and Synapsin).
Polyclonal Sheep IgG
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S778 of Dynamin
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Detection of Phospho-Dynamin (S778) by Western Blot.
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at S778 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-Dynamin (S778). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Rat dynamin (or Dynamin 1), also known as Dephosphin, is a 95 -100 kDa GTPase that belongs to the "classic dynamin" group of the dynamin superfamily. It is 864 amino acids (aa) in length, and contains three N-terminal GTP-binding elements (aa 38-208), one pleckstrin-homology domain (aa 519-625), a GED (GTPase Effector Domain) (aa 645-745), and a C-terminal SH3-binding proline-rich region (aa 753-864). At least four alternate splice forms are known, one that shows a 47 aa substitution between aa 398-445, and three that generate unique C-termini over the last 20 amino acids. Dynamin is found constitutively phosphorylated and inactive in the cytoplasm just under synaptic membranes. Upon stimulation, calcineurin dephosphorylates, and nitric oxide nitrosylates, Dynamin. Dephosphorylated and nitrosylated Dynamin now interacts with the cell membrane, generating endocytic synaptic vesicles. The process terminates when Cdk5 rephosphorylates dynamin on S744 and S778.
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