Phospho-GAP‑43 (S41) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Phospho-GAP‑43 (S41) by Western blot. Western blot of rat cortex lysate showing specific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at S41 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GAP-43 (S41). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
GAP-43 (Growth Associated Protein 43 kDa), also known as Neuromodulin, is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.
Citation for Phospho-GAP‑43 (S41) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis
Authors: IC Frei, D Weissenber, D Ritz, W Heusermann, M Colombi, M Shimobayas, MN Hall
Molecular Metabolism, 2022;0(0):101580.
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