Phospho-PLKK (S482/486/490) Antibody

R&D Systems | Catalog # PPS034

R&D Systems
Discontinued Product
PPS034 has been discontinued. View all PLKK products.

Key Product Details

Species Reactivity

Human, Mouse, Rat, Xenopus, Zebrafish

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PLKK Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S482/486/490 of Xenopus PLKK

Specificity

Human, mouse, rat, zebrafish, and Xenopus ~120 kDa PLKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Phospho-PLKK (S482/486/490) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Xenopus lysates

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: PLKK

Xenopus Polo-Like Kinase Kinase (xPLKK1) is a 120 kDa Ser/Thr kinase that is associated with 67 kDa PLx1 (Polo-Like Xenopus Kinase 1). PLx1 is involved with spindle assembly and centrosome maturation during the late G2 and M cell cycle phase. A cell’s entry into mitosis is controlled by the activation of Cyclin B/cdc2. cdc2 is constitutively phosphorylated and inactive, and activated by dephosphorylation of Tyr at position 15. The phosphatase that accomplishes this is cdc25C, which is activated by phosphorylation at multiple sites by a polo-like kinase termed PLx1 (PLK1 in mammals). The activating enzyme that acts on PLx1 is under investigation, and the kinase termed xPLKK1 has been identified as a candidate. xPLKK1 is 950 amino acids (aa) in size and contains a 264 amino acid kinase domain (aa 31 - 294). It contains three serines within a nine amino acid span (S482/486/490) which, when phosphorylated, activate the enzyme. Activation of PLx1 appears to depend on the phosphorylation of T201. And while xPLKK1 phosphorylates PLx1, it does so only on T10. Thus, its role in PLx1 activation is unclear. Notably, PLx1 has been shown to activate xPLKK1 on S482/486/490, suggesting that xPLKK1 may actually be a downstream target of PLx1 rather than an activator of PLx1.

References

  1. Lowery, D.M. et al. (2004) Cell Cycle 3:128.
  2. Erickson, E. et al. (2004) J. Biol. Chem. 279:32219.
  3. Qian, Y-W. et al. (1998) Science 282:1701.
  4. Kelm, O. et al. (2002) J. Biol. Chem. 277:25247.

Long Name

Polo-like Kinase Kinase

Alternate Names

PLK, STPK13

Entrez Gene IDs

5347 (Human)

Gene Symbol

PLK1

Product Documents for Phospho-PLKK (S482/486/490) Antibody

Certificate of Analysis

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Product Specific Notices for Phospho-PLKK (S482/486/490) Antibody

For research use only

Related Research Areas

Intracellular Kinases

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