Recombinant Human PIN4 His Protein

Novus Biologicals | Catalog # NBP1-51068

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
View all PIN4 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-156 of Human PIN4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PIN4 His Protein

SDS-PAGE: Recombinant Human PIN4 His Protein [NBP1-51068]

SDS-PAGE: Recombinant Human PIN4 His Protein [NBP1-51068]

SDS-Page: Recombinant Human PIN4 Protein [NBP1-51068] - 15 % SDS-PAGE (3ug)

Formulation, Preparation, and Storage

NBP1-51068
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 0.1 mM PMSF
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIN4

PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

4 (parvulin), MGC138486, Parvulin-17, protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)

Gene Symbol

PIN4

Additional PIN4 Products

Product Documents for Recombinant Human PIN4 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PIN4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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