PKC ζ pseudosubstrate

Biological Activity

PKC ζ pseudosubstrate is an inhibitor of protein kinase C (PKC) ζ; attached to cell permeabilisation Antennapedia domain vector peptide. Consists of amino acids 113 - 129 of PKC ζ pseudosubstrate domain linked by a disulphide bridge to the Antennapedia domain vector peptide. The Antennapedia peptide is actively taken up by intact cells, at 4 or 37°C, ensuring rapid and effective uptake of the inhibitor peptide. Once inside the cell, the disulphide bonds are subjected to reduction in the cytoplasm leading to release of the inhibitor peptide. Induces mast cell degranulation by a PKC ζ-independent pathway.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. TGFbeta-induced phosphorylation of Par6 promotes migration and invasion in prostate cancer cells.
   Mu Y, Zang G, Engstrom U, Busch C, Landstrom M
   Br J Cancer, 2015;112(7):1223-31.
2. A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity.
   Lim et al.
   Life Sci., 2008;82:733
3. Evidence of ζ protein kinase C involvement in polymorphonuclear neutrophil integrin-dependent adhesion and chemotaxis.
   Laudanna et al.
   J.Biol.Chem, 1998;273:30306
4. Intraneuronal delivery of protein kinase C pseudosubstrate leads to growth cone collapse.
   Theodore et al.
   J.Neurosci., 1995;15:7158
5. Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis
   RJ Pickering, C Tikellis, CJ Rosado, D Tsorotes, A Dimitropou, M Smith, O Huet, RM Seeber, R Abhayaward, EK Johnstone, J Golledge, Y Wang, KA Jandeleit-, ME Cooper, KD Pfleger, MC Thomas
   J. Clin. Invest., 2019;129(1):406-421.

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