POMT2 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP1-86152PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Applications

Antibody Competition
View all POMT2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human POMT2.

Source: E. coli

Amino Acid Sequence: GDGFFSSAFQARLSGNNLHNASIPEHLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNTNSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPMTRKHYQVTGYGINGT

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-86152.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP1-86152PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: POMT2

POMT2, also known as Protein-O-Mannosyltransferase 2, is an O-mannosyltransferase that must interact with POMT1 for proper enzymatic function. POMT2 is localized to the Endoplasmic Reticulum membrane, and is ubiquitously expressed at low levels, however highly expressed in the testes. Current research surrounding POMT2 has shown a connection with autosomal recessive muscular dystrophy, as well as possible interactions with Walker-Warburg syndrome, cleft palate, and malaria. POMT2 interacts with POMT1, ALG3, ALG6, ARPC3, and ATP13A1.

Alternate Names

DKFZp686G10254, Dolichyl-phosphate-mannose--protein mannosyltransferase 2, EC 2.4.1.109, FLJ22309, MDDGA2, MDDGB2, MDDGC2, protein O-mannosyl-transferase 2, protein-O-mannosyltransferase 2

Gene Symbol

POMT2

Additional POMT2 Products

Product Documents for POMT2 Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for POMT2 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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